SFM: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
{DISPLAYTITLE: MS or SFM} | |||
=Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium= | |||
Soya Flour Mannitol (SFM) medium, also called Mannitol Soya Flour (MS) medium, is a complex medium used for the routine cultivation of ''Streptomyces'' sp. Most will sporulate well on this medium. | |||
==Preparation== | ==Preparation== | ||
Per Litre: | Per Litre: | ||
*20 g Mannitol | *20 g Mannitol | ||
*20 g | *20 g Soya Flour | ||
*20 g Agar | *20 g Agar | ||
*1 L tap water | *1 L tap water | ||
| Line 12: | Line 14: | ||
'''Instructions''' | '''Instructions''' | ||
#Dissolve 20g mannitol in 1L tap water | #Dissolve 20g mannitol in 1L tap water | ||
#Add 2g | #Add 2g soya flour and 2g agar to each 250mL flask | ||
#Add 100mL mannitol solution to each flask | #Add 100mL mannitol solution to each flask | ||
#Autoclave | #Autoclave | ||
| Line 24: | Line 26: | ||
Soya flour is available from many health food shops and supermarkets (it is not necessary to purchase the more expensive material from a laboratory supplier). | Soya flour is available from many health food shops and supermarkets (it is not necessary to purchase the more expensive material from a laboratory supplier). | ||
Liquid SFM medium may be made by omitting the agar. | Liquid MS/SFM medium may be made by omitting the agar. | ||
Kieser et al (2000) recommend autoclaving SFM twice. Though this is not strictly necessary, the second autoclave cycle can help to break down some of the complex carbohydrates present in the soy flour, making them more readily available to the Streptomyces. Thus, you may observe improved growth and/or development when growing your strains on 2x autoclaved SFM. | Kieser et al (2000) recommend autoclaving MS/SFM twice. Though this is not strictly necessary, the second autoclave cycle can help to break down some of the complex carbohydrates present in the soy flour, making them more readily available to the Streptomyces. Thus, you may observe improved growth and/or development when growing your strains on 2x autoclaved MS/SFM. | ||
''[[Streptomyces venezuelae]]'' and ''[[Streptomyces clavuligerus]]'' do not sporulate well on SFM medium. | ''[[Streptomyces venezuelae]]'' and ''[[Streptomyces clavuligerus]]'' do not sporulate well on MS/SFM medium. | ||
SFM may also be used for bioassays, especially if supplemented with e.g. [[N-acetylglucosamine]] or [[sodium butyrate]] to elicit antibiotic production. | MS/SFM may also be used for bioassays, especially if supplemented with e.g. [[N-acetylglucosamine]] or [[sodium butyrate]] to elicit antibiotic production. | ||
==References== | ==References== | ||
Revision as of 09:57, 30 July 2019
{DISPLAYTITLE: MS or SFM}
Mannitol Soya Flour (MS) a.k.a. Soya Flour Mannitol (SFM) Medium
Soya Flour Mannitol (SFM) medium, also called Mannitol Soya Flour (MS) medium, is a complex medium used for the routine cultivation of Streptomyces sp. Most will sporulate well on this medium.
Preparation
Per Litre:
- 20 g Mannitol
- 20 g Soya Flour
- 20 g Agar
- 1 L tap water
Instructions
- Dissolve 20g mannitol in 1L tap water
- Add 2g soya flour and 2g agar to each 250mL flask
- Add 100mL mannitol solution to each flask
- Autoclave
Uses
- Routine growth of Streptomyces sp.
- Conjugations
- Preparing spore stocks
Notes
Soya flour is available from many health food shops and supermarkets (it is not necessary to purchase the more expensive material from a laboratory supplier).
Liquid MS/SFM medium may be made by omitting the agar.
Kieser et al (2000) recommend autoclaving MS/SFM twice. Though this is not strictly necessary, the second autoclave cycle can help to break down some of the complex carbohydrates present in the soy flour, making them more readily available to the Streptomyces. Thus, you may observe improved growth and/or development when growing your strains on 2x autoclaved MS/SFM.
Streptomyces venezuelae and Streptomyces clavuligerus do not sporulate well on MS/SFM medium.
MS/SFM may also be used for bioassays, especially if supplemented with e.g. N-acetylglucosamine or sodium butyrate to elicit antibiotic production.
References
Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK
Hobbs, G, Frazer, CM, Gardner, DCJ, Cullum, JA & Oliver, SG 1989, 'Dispersed growth of Streptomyces in liquid culture', Applied microbiology and biotechnology, vol. 31, no. 3, pp. 272-277. https://doi.org/10.1007/BF00258408
