SNA: Difference between revisions
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=SNA= | =Soft Nutrient Agar (SNA)= | ||
Soft Nutrient Agar (SNA) is a modified version of [[DNA]] containing half the amount of agar. It is commonly used for overlays containing motile organisms, and for plaquing bacteriophage. | |||
==Preparation== | ==Preparation== | ||
| Line 18: | Line 19: | ||
==Uses== | ==Uses== | ||
*[[Bioassays]] | *[[Bioassays]] | ||
*[[Streptomyces Bacteriophage plaque assay]] | |||
Latest revision as of 12:52, 5 May 2020
Soft Nutrient Agar (SNA)
Soft Nutrient Agar (SNA) is a modified version of DNA containing half the amount of agar. It is commonly used for overlays containing motile organisms, and for plaquing bacteriophage.
Preparation
Per Litre:
- 4g Difco Nutrient Broth Powder
- 5g Agar
- 1L dH2O
Instructions
- Add 0.8g of DNB powder into each 250ml conical flask
- Add 1g of Agar
- Add 200ml dH2O
- Autoclave
Uses
Notes
References
Kieser, T., Bibb, M.J., Buttner, M.J., Chater, K.F. and Hopwood, D.A. (2000) Practical Streptomyces Genetics: John Innes Foundation, Norwich Research Park, Colney, Norwich NR4 7UH, UK
