All public logs
From ActinoBase
Combined display of all available logs of ActinoBase. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 15:31, 20 February 2020 User account GenomeBrowser talk contribs was created by Sam Prudence talk contribs
- 15:26, 20 February 2020 Sam Prudence talk contribs moved page Salinospora to Salinispora
- 15:22, 20 February 2020 Sam Prudence talk contribs created page Genome browser (Created page with "<h2><strong>ActinoBase Genome Browser</strong></h2> Click the link below for the ActinoBase MORF Genome Browser, with antiSMASH integration. This tool has been kindly provide...")
- 19:50, 16 February 2020 JoshKlein talk contribs created page File:BioArtElvis.PNG (BioArt photo from https://doi.org/10.1371/journal.pbio.1000510.)
- 19:50, 16 February 2020 JoshKlein talk contribs uploaded File:BioArtElvis.PNG (BioArt photo from https://doi.org/10.1371/journal.pbio.1000510.)
- 19:49, 16 February 2020 JoshKlein talk contribs created page File:BioArt.png (A picture of various BioArt plates.)
- 19:49, 16 February 2020 JoshKlein talk contribs uploaded File:BioArt.png (A picture of various BioArt plates.)
- 15:27, 31 January 2020 ElmiraM talk contribs created page Streptacidiphilus (Created page with "=<i>Streptacidiphilus</i>= Streptacidiphilus (etymology: twisted, acid-loving) are one of the three genera belonging to the <i>Streptomycetaceae<i> family, along with <i>Strep...")
- 15:23, 31 January 2020 MFeeney talk contribs created page Lambda-red mediated recombination using ssDNA (Created page with "=Lambda-red mediated recombination using ssDNA= Lambda-red mediated recombination using ssDNA can be used to introduce precise mutations into the genome of <em>Streptomyces</e...")
- 14:47, 31 January 2020 Jordanmurray talk contribs created page Cultivating Actinobacteria for Microscopic Analysis (Created page with "In order to analyse actinobacteria using light microscopy, methods must take into account the multicellular nature of the test organism. Unlike unicellular organisms, actinoba...")
- 16:31, 29 January 2020 R Devine talk contribs created page ChIP Sequencing (Created page with "==General overview== Chromatin Immunoprecipitation and sequencing can be used to characterise protein-DNA interactions. It works by cross-linking proteins to DNA, shearing t...")
- 16:56, 14 November 2019 Sam Prudence talk contribs deleted page Trace elements (content was: "=Trace Elements= Recipe not added", and the only contributor was "Test" (talk))
- 17:29, 7 November 2019 Sam Prudence talk contribs deleted page CRISPR (content before blanking was: "Hutchings Lab protocol for generating CRISPR/Cas Knockouts using pCRISPomyces-2. Written by Rebecca Devine, Matt Hutchings’ Laboratory, University of East Anglia, UK <strong>Overview (see following pages for more deta...")
- 14:19, 25 October 2019 DaveMark talk contribs created page Isolating Actinobacteria from soil environments (Created page with "Isolating Actinobacteria from soil environments =Equipment & Materials= *Sterile containers to transport the soil *A heat block *Plates of Isolation media *A vortexer *¼ Str...")
- 14:08, 25 October 2019 LeightonP talk contribs created page Using pandoc (add page describing use of pandoc to convert Word documents to MediaWiki format)
- 14:01, 25 October 2019 LeightonP talk contribs created page File:Example word file.png (Screenshot of example Word file, before pandoc conversion)
- 14:01, 25 October 2019 LeightonP talk contribs uploaded File:Example word file.png (Screenshot of example Word file, before pandoc conversion)
- 13:17, 25 October 2019 User account CharlieBegley talk contribs was created by DaveMark talk contribs
- 13:16, 25 October 2019 User account MegJeppesen talk contribs was created by DaveMark talk contribs (New Member)
- 13:15, 25 October 2019 User account MollyKeithBaker talk contribs was created by DaveMark talk contribs (New Member)
- 13:14, 25 October 2019 User account ElmiraM talk contribs was created by DaveMark talk contribs
- 15:16, 17 October 2019 Alicia talk contribs created page Site-specific phage recombinase (Created page with "<strong>Integration sites</strong> You can check the sequence of your organism to see if it contains the appropriate integration site for your chosen plasmid: *ΦBT1, attB...")
- 15:44, 10 October 2019 J Newitt talk contribs created page Links master page (Created page with "=Useful Links= ==Bioinformatics== ===16S rRNA metaprofiling=== [https://greengenes.secondgenome.com Green Genes] 16S rRNA gene database [https://www.arb-silva.de/ SILVA]...")
- 16:25, 30 September 2019 DaveMark talk contribs created page Aminoglycosides (Created page with "=Intro= The aminoglycoside antibiotics are a class of amino containing oligosaccharides, and are notable for their broad-spectrum bacteriocidal activity - including their abil...")
- 14:35, 27 September 2019 LisAlgora talk contribs created page Southern Blotting (Created page with "There are many different protocols for Southern blotting. This approach uses vacuum transfer pf DNA to a nylon membrane, DIG-labelling of the probe and detection by chromogeni...")
- 13:53, 27 September 2019 EmilyAddington talk contribs created page Actinomycetes Morphology and Lifecycle (Created blank page)
- 08:57, 27 September 2019 User account JoshKlein talk contribs was created by MFeeney talk contribs and password was sent by email
- 08:56, 27 September 2019 User account LouCharkoudian talk contribs was created by MFeeney talk contribs and password was sent by email
- 16:01, 26 September 2019 J Newitt talk contribs created page General Growth and Maintenance of Streptomyces sp. (Created page with "=General growth and maintenance of ''Streptomyces'' species= ''Streptomyces'' are multi-nuclear filamentous organisms that undergo several different stages of growth. This se...")
- 15:25, 26 September 2019 Tommclean talk contribs created page Luria (Created page with "=Luria Broth= Known as Luria Broth (or sometimes, mistakenly, as LB or Lennox medium), this rich medium is used routinely for growth of <em>Escherichia coli</em>. There are se...")
- 15:25, 26 September 2019 Sam Prudence talk contribs created page File:SCOR2.jpg
- 15:25, 26 September 2019 Sam Prudence talk contribs uploaded File:SCOR2.jpg
- 15:23, 26 September 2019 Tommclean talk contribs created page Lennox (Created page with "=Lennox Broth= Known as Lennox Broth (or sometimes, mistakenly, as Luria-Bertani medium), this rich medium is used routinely for growth of <em>Escherichia coli</em>. There are...")
- 15:12, 26 September 2019 Tommclean talk contribs created page R5 (Created page with "=R5= ==Preparation== Make up the following solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *10.12 g MgCl<sub>2</sub>.6H<sub>2</sub>O *10 g Glucose *0.1 g Difco...")
- 15:57, 12 September 2019 Sam Prudence talk contribs created page Isolating extracellular protein from Streptomyces (Created page with " =Isolation of extracellular protein from ''Streptomyces''= This protocol can be used to isolate membrance associated and secreted extracellular proteins from ''Streptomyces''...")
- 09:15, 11 September 2019 Sam Prudence talk contribs created page Test (Created page with "| rowspan="8" valign="top" width="120" | texty wexty ! colspan="2" align="left" | <font face="Arial Black" size="5" style="text-transform: uppercase;"> Heading A </font> | row...")
- 13:20, 4 September 2019 LisAlgora talk contribs created page Trace element solution (Created page with "'''Trace element solution (per liter):''' *40 mg ZnCl<sub>2</sub> *200 mg FeCl<sub>3</sub> . 6H<sub>2</sub>O *10 mg CuCl<sub>2</sub> . 2H<sub>2</sub>O *10 mg MnCl<sub>2</sub>...")
- 13:17, 4 September 2019 LisAlgora talk contribs created page L buffer (Created page with "'''L (lysis) buffer''' (Thompson ''et al.'' 1982) Mix the following sterile solutions: *100 ml Sucrose (10.3%) *10 ml TES buffer (5.73%, adjusted to pH7.2) *1 ml K<sub>2</su...")
- 12:51, 4 September 2019 LisAlgora talk contribs created page P buffer (Created page with "'''P (protoplast) buffer''' (Okanishi ''et al.'', 1974; Hopwood and Wright, 1978) Make up the following basal solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *2....")
- 09:15, 4 September 2019 User account D Guillen talk contribs was created by Sam Prudence talk contribs
- 14:37, 2 September 2019 User account LeightonP talk contribs was created by MFeeney talk contribs and password was sent by email
- 04:37, 31 August 2019 H Otani talk contribs created page Rhodococcus jostii (Created page with "==Organism Page== === ''Rhodococcus jostii'' === ===Background=== ===Growth & Maintenance=== LB or M9 =====Sporulation===== Nonsporulating ===Strains=== <em>Rh...")
- 00:21, 31 August 2019 H Otani talk contribs created page Rhodococcus (Created page with "Rhodococci are nonsporulating actinomycetes in the family Nocardiaceae along with <em>Nocardia</em>. They are known for their ability to catabolise a wide range of organic...")
- 20:48, 30 August 2019 H Otani talk contribs created page Saccharopolyspora spinosa (Created page with "==Organism Page== === ''Saccharopolyspora spinosa'' === ===Background=== <em>Saccharopolyspora spinosa</em> is known for producing an insecticide, spinosad. Spinosad is a m...")
- 20:37, 30 August 2019 H Otani talk contribs created page YMPD (Created page with "=YMPD (Yeast Meat Peptone Dextrose)= For growth of ''Streptomyces griseus''. ==Preparation== Per litre: *2 g yeast extract *2.2 g meat extract *4 g Bacto peptone *5 g Na...")
- 15:40, 30 August 2019 EmilyAddington talk contribs uploaded a new version of File:Lifecycle.jpeg
- 15:36, 30 August 2019 EmilyAddington talk contribs created page File:Lifecycle.jpeg
- 15:36, 30 August 2019 EmilyAddington talk contribs uploaded File:Lifecycle.jpeg
- 15:28, 30 August 2019 LisAlgora talk contribs created page Protoplasts Formation, Regeneration and Transformation (Created page with "Protoplasts are used for fusion, transformation and transfection. =Protoplasts Preparation= Although this protocol was written for ''Streptomyces lividans'' and ''Str...")
- 15:03, 30 August 2019 ASBirke talk contribs created page Streptomyces clavuligerus (Created page with "=''Streptomyces clavuligerus''= ''Streptomyces clavuligerus'' was originally isolated from a South American soil sample for its ability to produce cephalosporins. Tod...")