User contributions for LisAlgora
From ActinoBase
23 April 2020
- 22:3722:37, 23 April 2020 diff hist +1,267 N Lambda DNA/HindIII Ladder Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate..." current
- 22:3622:36, 23 April 2020 diff hist 0 Southern Blotting →2. PCR Labelling current
- 22:3522:35, 23 April 2020 diff hist +1,267 N Lambda DNA/HindIII ladder Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate..." current
- 22:2322:23, 23 April 2020 diff hist +8,185 N Solutions for Southern Blotting Created page with "=Stock Solutions to Prepare in Advance= {| class="wikitable" ! style="font-family:Arial, Helvetica, sans-serif !important;; background-color:rgb(222, 234, 246); color:rgb(0,..." current
- 21:5521:55, 23 April 2020 diff hist +4,607 N Consumables for Southern Blotting Created page with "Roche provides a <b>DIG DNA Labelling and Detection Kit</b> that contains: blocking reagent, Anti-DIG-AP conjugate, Hexanucleotide Mix, DIG DNA Labelling Mix, Klenow Enzyme an..." current
- 21:1921:19, 23 April 2020 diff hist +6,785 Southern Blotting No edit summary
27 September 2019
- 14:3514:35, 27 September 2019 diff hist +2,947 N Southern Blotting Created page with "There are many different protocols for Southern blotting. This approach uses vacuum transfer pf DNA to a nylon membrane, DIG-labelling of the probe and detection by chromogeni..."
- 13:4513:45, 27 September 2019 diff hist +23 Nucleic Acid Methods No edit summary current
4 September 2019
- 14:1314:13, 4 September 2019 diff hist +4 Streptomyces clavuligerus No edit summary
- 14:1114:11, 4 September 2019 diff hist +2,279 Streptomyces clavuligerus No edit summary
- 13:2713:27, 4 September 2019 diff hist −358 R2YE No edit summary current
- 13:2513:25, 4 September 2019 diff hist +2 P buffer No edit summary current
- 13:2313:23, 4 September 2019 diff hist −24 P buffer No edit summary
- 13:2113:21, 4 September 2019 diff hist −2 R2 No edit summary
- 13:2113:21, 4 September 2019 diff hist +2 L buffer No edit summary current
- 13:2013:20, 4 September 2019 diff hist +341 N Trace element solution Created page with "'''Trace element solution (per liter):''' *40 mg ZnCl<sub>2</sub> *200 mg FeCl<sub>3</sub> . 6H<sub>2</sub>O *10 mg CuCl<sub>2</sub> . 2H<sub>2</sub>O *10 mg MnCl<sub>2</sub>..."
- 13:1813:18, 4 September 2019 diff hist −356 R2 No edit summary
- 13:1713:17, 4 September 2019 diff hist +516 N L buffer Created page with "'''L (lysis) buffer''' (Thompson ''et al.'' 1982) Mix the following sterile solutions: *100 ml Sucrose (10.3%) *10 ml TES buffer (5.73%, adjusted to pH7.2) *1 ml K<sub>2</su..."
- 12:5112:51, 4 September 2019 diff hist +513 N P buffer Created page with "'''P (protoplast) buffer''' (Okanishi ''et al.'', 1974; Hopwood and Wright, 1978) Make up the following basal solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *2...."
- 12:4012:40, 4 September 2019 diff hist +60 Cloning, Transformation and Expression of Gene Clusters No edit summary current