All public logs
From ActinoBase
Combined display of all available logs of ActinoBase. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 22:37, 23 April 2020 LisAlgora talk contribs created page Lambda DNA/HindIII Ladder (Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate...")
- 22:35, 23 April 2020 LisAlgora talk contribs created page Lambda DNA/HindIII ladder (Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate...")
- 22:23, 23 April 2020 LisAlgora talk contribs created page Solutions for Southern Blotting (Created page with "=Stock Solutions to Prepare in Advance= {| class="wikitable" ! style="font-family:Arial, Helvetica, sans-serif !important;; background-color:rgb(222, 234, 246); color:rgb(0,...")
- 21:55, 23 April 2020 LisAlgora talk contribs created page Consumables for Southern Blotting (Created page with "Roche provides a <b>DIG DNA Labelling and Detection Kit</b> that contains: blocking reagent, Anti-DIG-AP conjugate, Hexanucleotide Mix, DIG DNA Labelling Mix, Klenow Enzyme an...")
- 14:35, 27 September 2019 LisAlgora talk contribs created page Southern Blotting (Created page with "There are many different protocols for Southern blotting. This approach uses vacuum transfer pf DNA to a nylon membrane, DIG-labelling of the probe and detection by chromogeni...")
- 13:20, 4 September 2019 LisAlgora talk contribs created page Trace element solution (Created page with "'''Trace element solution (per liter):''' *40 mg ZnCl<sub>2</sub> *200 mg FeCl<sub>3</sub> . 6H<sub>2</sub>O *10 mg CuCl<sub>2</sub> . 2H<sub>2</sub>O *10 mg MnCl<sub>2</sub>...")
- 13:17, 4 September 2019 LisAlgora talk contribs created page L buffer (Created page with "'''L (lysis) buffer''' (Thompson ''et al.'' 1982) Mix the following sterile solutions: *100 ml Sucrose (10.3%) *10 ml TES buffer (5.73%, adjusted to pH7.2) *1 ml K<sub>2</su...")
- 12:51, 4 September 2019 LisAlgora talk contribs created page P buffer (Created page with "'''P (protoplast) buffer''' (Okanishi ''et al.'', 1974; Hopwood and Wright, 1978) Make up the following basal solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *2....")
- 15:28, 30 August 2019 LisAlgora talk contribs created page Protoplasts Formation, Regeneration and Transformation (Created page with "Protoplasts are used for fusion, transformation and transfection. =Protoplasts Preparation= Although this protocol was written for ''Streptomyces lividans'' and ''Str...")
- 13:53, 30 August 2019 LisAlgora talk contribs created page File:PIJ6902.gif
- 13:53, 30 August 2019 LisAlgora talk contribs uploaded File:PIJ6902.gif
- 13:48, 30 August 2019 LisAlgora talk contribs created page PIJ6902 (Created page with "{{DISPLAYTITLE: pIJ6092}} ==Use== ==Features== *Apramycin resistance cassette *φBT1 integration site & integrase ==History== ==Map== ==Sequence links== ==References=...")
- 17:16, 26 July 2019 LisAlgora talk contribs created page R2YE (Created page with "=R2YE= R2YE medium is a variation of R2 containing yeast extract. ==Preparation== Make up the following solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *10.12 g...")
- 16:01, 26 July 2019 LisAlgora talk contribs created page R2 (Created page with "=R2= ==Preparation== #Make up the following solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *10.12 g MgCl<sub>2</sub>.6H<sub>2</sub>O *10 g Glucose *0.1 g Difc...")
- 14:46, 26 July 2019 LisAlgora talk contribs created page SNA (Created page with "=SNA= Soft Nutrient Agar (SNA) ==Preparation== Per Litre: *4g Difco Nutrient Broth Powder *5g Agar *1L dH<sub>2</sub>O '''Instructions''' #Add 0.8g of DNB powder into eac...")
- 14:24, 26 July 2019 LisAlgora talk contribs created page 2X YT (Created page with "=2x YT= ==Preparation== Per litre: *16 g Tryptone *10 g Yeast Extract *5 g NaCl *1 L dH<sub>2</sub>O '''Instructions''' #Dissolve constituents in 1L dH<sub>2</sub>O #Ste...")