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• 11:00, 2 March 2026 Matt Hutchings talk contribs uploaded File:PSS150.png
• 11:00, 2 March 2026 Matt Hutchings talk contribs created page File:PSS150.png
• 10:45, 2 March 2026 Matt Hutchings talk contribs created page PSS150 (Created page with "pSS150: pIJ10257 carrying PermE*-sfgfp, HygR Codon-optimised sfgfp was inserted between the NdeI/XhoI of pIJ10257 Reference: Casu B, Sallmen JW, Schlimpert S, Pilhofer M. Cytoplasmic contractile injection systems mediate cell death in Streptomyces. Nat Microbiol. 2023 Apr;8(4):711-726. doi: 10.1038/s41564-023-01341-x. Epub 2023 Mar 9. PMID: 36894633; PMCID: PMC10066040.")
• 12:39, 27 January 2026 Matt Hutchings talk contribs created page ABB13 (Created page with "ABB13 is a great medium for inducing antibiotic production and has also been used to improve transformation efficiency in actinomycetes. ABB1 base medium contained the following components (g/L): 10g Bacto Soytone, 9g yeast extract, 10g soluble starch, 15g cerelose Filter sterilised 3-N-morpholinopropanesulfonic acid (MOPS) buffer, pH 7.0, was added to ABB1 base medium after autoclaving to a final concentration of 50 mM. <strong>References</strong> Fitzgerals et al...")
• 15:48, 14 November 2025 User account KNoble talk contribs was created by Matt Hutchings talk contribs (Editor)
• 12:01, 4 November 2025 Matt Hutchings talk contribs created page YMS (Created page with "Yeast extract: 4.0 g Malt extract: 10.0 g Soluble starch: 10.0 g (or sometimes as high as 30.0 g) Agar: 20.0 g (for solid medium) Calcium carbonate: 2.0 g (often added after adjusting pH) Distilled water: 1000 mL ")
• 10:44, 13 January 2025 Matt Hutchings talk contribs blocked Sam Prudence talk contribs with an expiry time of indefinite (account creation disabled)
• 10:44, 13 January 2025 Matt Hutchings talk contribs blocked S Worsley talk contribs with an expiry time of indefinite (account creation disabled)
• 10:44, 13 January 2025 Matt Hutchings talk contribs blocked N Som talk contribs with an expiry time of indefinite (account creation disabled)
• 10:43, 13 January 2025 Matt Hutchings talk contribs blocked Louisa Perry talk contribs with an expiry time of indefinite (account creation disabled)
• 10:43, 13 January 2025 Matt Hutchings talk contribs blocked Louisa perry talk contribs with an expiry time of indefinite (account creation disabled)
• 10:43, 13 January 2025 Matt Hutchings talk contribs blocked Jacob Hamilton talk contribs with an expiry time of indefinite (account creation disabled)
• 10:42, 13 January 2025 Matt Hutchings talk contribs blocked Alicia talk contribs with an expiry time of indefinite (account creation disabled)
• 10:42, 13 January 2025 Matt Hutchings talk contribs blocked Made talk contribs with an expiry time of indefinite (account creation disabled)
• 10:41, 13 January 2025 Matt Hutchings talk contribs blocked Andyxmas talk contribs with an expiry time of indefinite (account creation disabled)
• 10:41, 13 January 2025 Matt Hutchings talk contribs blocked Test talk contribs with an expiry time of indefinite (account creation disabled)
• 10:40, 13 January 2025 Matt Hutchings talk contribs blocked Louisa perry jic talk contribs with an expiry time of indefinite (account creation disabled)
• 10:39, 13 January 2025 Matt Hutchings talk contribs blocked Damien Gayrard talk contribs with an expiry time of indefinite (account creation disabled)
• 10:34, 13 January 2025 User account Gageowens talk contribs was created by Matt Hutchings talk contribs (New editor)
• 09:41, 29 November 2024 Matt Hutchings talk contribs uploaded File:PIJ86.jpg
• 09:41, 29 November 2024 Matt Hutchings talk contribs created page File:PIJ86.jpg
• 10:15, 28 November 2024 Matt Hutchings talk contribs created page PIJ86 (Created page with "Plasmid sequence of pIJ86 is [here https://www.ncbi.nlm.nih.gov/nuccore/2831519705]")
• 11:07, 14 November 2024 Matt Hutchings talk contribs created page Bioinformatics Ressources (Created page with "===2024 SSAMM - Bioinformatics Tools and Online Resources=== ====Strains==== [http://streptomyces.org.uk Streptomyces.org] Links to resources including StrepDB and a free download of the Streptomyces manual. [https://bacdive.dsmz.de/ BacDive (The Bacterial Diversity Metadatabase) ] The world’s largest database for standardized bacterial information. Look up your strain, its morphology, culture and growth condition, DNA sequence accessions, chemotaxonomic features et...")
• 10:36, 14 November 2024 Matt Hutchings talk contribs created page Useful resources can be found here (Created page with "===2024 SSAMM - Bioinformatics Tools and Online Resources===")
• 10:33, 14 November 2024 Matt Hutchings talk contribs created page JIC Summer School in Applied Molecular Microbiology (Created page with "=== JIC Summer School in Applied Molecular Microbiology === Bionformatics Resources This summer school is organised by the John Innes Centre and is held every two years in Dubrovnik, hosted by the [https://iuc.hr/ IUC Dubrovnik]. Typically we host around 50 students (typically PhD students and postdocs) and the course runs over eight days. Info on the 2024 summer school can be found [https://www.jic.ac.uk/training-careers/summ...")
• 10:01, 14 November 2024 Matt Hutchings talk contribs created page JIC Summer School in Applied Microbiology (Created page with "JIC Summer School in Applied Molecular Microbiology This summer school is organised by the John Innes Centre and is held every two years in Dubrovnik, hosted by the [https://iuc.hr/] IUC Dubrovnik. Typically we host around 50 students (typically PhD students and postdocs) an the course runs over eight days. Info on the 2024 summer school can be found [https://www.jic.ac.uk/training-careers/summer-schools/applied-molecular-microbiology/ here]")
• 09:06, 31 July 2024 Matt Hutchings talk contribs created page PIJ6021 (Created page with "Plasmid pIJ6021 allows for high level protein production in ''Streptomyces'' species. This plasmid was first described by [https://www.sciencedirect.com/science/article/pii/0378111995005452?via%3Dihub Takano et al 1995] and utilises the pIJ101 origin of replication which leads to high copy number, sometimes approaching 100 copies per cell. It includes the thiostrepton inducible ''tipA'' promoter to induce expression of the target gene.")
• 08:55, 31 July 2024 Matt Hutchings talk contribs created page PUZ8002 (Created page with "This is a helper plasmid that encodes tha ,achnery required for conjugating other plasmids into 'Streptomyces'. For everything you ever needed to know about pUZ8002 please read this paper: Larcombe et al, 2024. Sequence and origin of the Streptomyces intergenetic-conjugation helper plasmid pUZ8002. Access Micro 6:000808.v3. https://doi.org/10.1099/acmi.0.000808.v3")
• 12:43, 18 July 2024 Matt Hutchings talk contribs created page Protein production in Streptomyces (Created page with """Protocol for "Streptomyces" protein expression"" ""Background"" Plasmid pIJ6021 allows for high level protein production in "Streptomyces" species. This plasmid was first described by Bibb et al in 19951 and utilises the pIJ101 origin of replication which leads to high copy number, sometimes approaching 100 copies per cell. It includes the thiostrepton inducible tipA promoter to induce expression of the target gene. However, pIJ6021 lacks an E. coli replicon, meanin...")
• 09:27, 7 June 2024 Matt Hutchings talk contribs created page Streptomyces lividans (Created page with "== ''Streptomyces lividans'' 66(2) == ===Background=== <em>Streptomyces lividans</em> 66 (also known as 1326) is a model organism for <em>Streptomyces</em> research and particularly useful for heterologous production of proteins and small molecules since it does not restrict foreign methylated DNA.Thew most commonly used strais are TK23 and TK24 which are prototrophic and plasmid free. S. lividans strains 66 and TK24 have both been genome sequenced. ===Growth & Mai...")
• 12:00, 12 March 2024 User account Annie talk contribs was created by Matt Hutchings talk contribs (New editor)
• 07:36, 11 October 2023 User account Gilles talk contribs was created by Matt Hutchings talk contribs
• 16:16, 12 September 2023 Matt Hutchings talk contribs undeleted page CRISPR (5 revisions)
• 15:59, 12 September 2023 Matt Hutchings talk contribs created page MtrA methods are here (Created page with "Som N, Devine R, Chandra G, Martins C, Wilkinson B and Hutchings MI (2023). MtrA regulates the expression of developmental genes, including BldM and WhiI, in Streptomyces vene...")
• 15:57, 12 September 2023 Matt Hutchings talk contribs created page Methods (Created page with "This allows you to publish supplementary methods to support, and cite, in your publications. Email matt.hutchings@jic.ac.uk Som N, Devine R, Chandra G, Martins C, Wilkinson...")
• 07:52, 21 August 2023 Matt Hutchings talk contribs created page VProtocols (Created page with "Below are links to video protocols created by our members: Streptomyces spore preparation by Lorena Fernández-Martínez <Fernandl@edgehill.ac.uk>")
• 09:18, 30 June 2023 User account Tomgreg talk contribs was created by Matt Hutchings talk contribs
• 07:45, 30 June 2023 User account Thomgregory talk contribs was created by Matt Hutchings talk contribs
• 08:18, 29 June 2023 User account Thomgreg talk contribs was created by Matt Hutchings talk contribs and password was sent by email (New editor)
• 16:38, 12 November 2021 User account Nelly talk contribs was created by Matt Hutchings talk contribs (New editor)
• 08:03, 21 October 2021 Matt Hutchings talk contribs created page Plant-Microbe interactions (Created page with "In recent years it has become clear that actinomycetes like Streptomyces species are not simply free-living soil bacteria. They also interact with plant roots and can colonise...")
• 07:53, 21 October 2021 Matt Hutchings talk contribs created page Plants (Created page with "150px|Protocols|link=Protocols =Protocols organised by category= ==Bacteriophage== *Streptomyces Bacteriophage plaque a...")