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From ActinoBase
Combined display of all available logs of ActinoBase. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 14:27, 26 July 2019 MFeeney talk contribs created page HV (Created page with "=HV= ==Preparation== Per Litre: *1g humic acid *1.7g KCl *0.5g Na<sub>2</sub>HPO<sub>4</sub> *0.5g MgSO<sub>4</sub> *0.02g CaCO<sub>3</sub> *0.01g FeSO<sub>4</sub> *1mL VB...")
- 14:24, 26 July 2019 LisAlgora talk contribs created page 2X YT (Created page with "=2x YT= ==Preparation== Per litre: *16 g Tryptone *10 g Yeast Extract *5 g NaCl *1 L dH<sub>2</sub>O '''Instructions''' #Dissolve constituents in 1L dH<sub>2</sub>O #Ste...")
- 14:18, 26 July 2019 DaveMark talk contribs created page ISP3 (Created page with "=ISP 3= International Streptomyces Project media 3 (ISP 3) is a rich general use media. Alternatively called oatmeal agar. ==Preparation== *20 g White Oats *18 g Agar *1 mL...")
- 14:18, 26 July 2019 Jordanmurray talk contribs created page Staining of Streptomyces spp. Cell Wall and Nucleic Acids (Created page with "The following staining technique is a useful method for visualising the actively growing regions of the cell wall, along with the nucleic acids within the cell. The method was...")
- 14:08, 26 July 2019 ActinoCommittee talk contribs created page Emerson medium (Created page with "=Emerson medium= Emerson medium is a rich medium formulated by Emerson et al (1) to isolate mould and Actinobacteria. It is composed of beef extract, yeast extract, peptic di...")
- 13:54, 26 July 2019 User account Jacob Hamilton talk contribs was created by Sam Prudence talk contribs
- 13:51, 26 July 2019 User account ASBirke talk contribs was created by MFeeney talk contribs
- 13:48, 26 July 2019 DaveMark talk contribs created page GYM (Created page with "=Glucose Yeast-Extract Malt-Extract Media= ==Preparation== Per litre: *4 g Glucose *4 g Yeast Extract *10 g Malt Extract *CaCo<sub>3</sub> *12 g Agar *1 L dH<sub>2</sub>O...")
- 13:47, 26 July 2019 User account REMcHugh talk contribs was created by MFeeney talk contribs
- 13:36, 26 July 2019 ActinoCommittee talk contribs moved page Environmental isolation to Actinobacteria isolation from marine environments
- 13:31, 26 July 2019 Jordanmurray talk contribs created page Microscopy (Created page with "=Microscopy of Actinobacteria= ==<em>Streptomyces</em>==")
- 13:25, 26 July 2019 User account RMcHugh talk contribs was created by MFeeney talk contribs
- 13:21, 26 July 2019 ActinoCommittee talk contribs created page Environmental isolation (Protocols to isolate Actinobacteria from different environments)
- 13:18, 26 July 2019 User account LisAlgora talk contribs was created by MFeeney talk contribs
- 13:17, 26 July 2019 User account Jordanmurray talk contribs was created by MFeeney talk contribs
- 13:00, 26 July 2019 User account MunnJ90 talk contribs was created by MFeeney talk contribs
- 11:48, 26 July 2019 MFeeney talk contribs created page Teaching and learning (Created page with "This is the hub for #ActinoBase teaching and learning resources: educational content, outreach, and more! We welcome contributions from the community, so please Contact us...")
- 16:06, 25 July 2019 User account Yvonne Mast talk contribs was created by Sam Prudence talk contribs
- 11:15, 24 July 2019 Alicia talk contribs created page Heterologous expression of gene clusters (Created page with "Gene clusters that have been cloned from their native host can be expressed heterologously in a different host organism. This can help improve production of known products, or...")
- 10:50, 24 July 2019 Alicia talk contribs created page PCAP03 (Created page with " The [https://pubs.acs.org/doi/10.1021/acschembio.5b00658 pCAP03 paper]")
- 08:51, 22 July 2019 Matth talk contribs created page CRISPR (Created page with "Hutchings Lab protocol for generating CRISPR/Cas Knockouts using pCRISPomyces-2. Written by Rebecca Devine, Matt Hutchings’ Laboratory, University of East Anglia, UK <em>O...")
- 20:10, 18 July 2019 Matth talk contribs created page CRISPR/Cas9-mediated genome editing (Created page with "pCRISPomyces2")
- 19:43, 17 July 2019 Matth talk contribs created page PSET152 (Created page with "File:pSET152.png")
- 19:43, 17 July 2019 Matth talk contribs created page File:PSET152.png
- 19:43, 17 July 2019 Matth talk contribs uploaded File:PSET152.png
- 19:40, 17 July 2019 Matth talk contribs created page File:PMS82.png
- 19:40, 17 July 2019 Matth talk contribs uploaded File:PMS82.png
- 19:39, 17 July 2019 Matth talk contribs created page PMS82 (Created page with "Integrating vector derived from the φBT1 attP-int locus and carrying the hygromycin resistance cassette. Described [https://jb.asm.org/content/185/17/5320.long here]")
- 19:27, 17 July 2019 Matth talk contribs created page PCRISPomyces2 (Created page with "file:CRISPR.png")
- 19:27, 17 July 2019 Matth talk contribs created page File:CRISPR.png
- 19:27, 17 July 2019 Matth talk contribs uploaded File:CRISPR.png
- 09:35, 5 July 2019 Matth talk contribs deleted page File:Timeline.png (Deleted old revision 20190705093521!Timeline.png)
- 09:35, 5 July 2019 Matth talk contribs uploaded a new version of File:Timeline.png
- 09:33, 5 July 2019 Matth talk contribs deleted page File:Timeline.png (Deleted old revision 20190705085928!Timeline.png)
- 09:33, 5 July 2019 Matth talk contribs deleted page File:Timeline.png (Deleted old revision 20190705093317!Timeline.png)
- 09:33, 5 July 2019 Matth talk contribs uploaded a new version of File:Timeline.png
- 08:59, 5 July 2019 Matth talk contribs uploaded a new version of File:Timeline.png
- 08:58, 5 July 2019 Matth talk contribs created page File:Timeline.png
- 08:58, 5 July 2019 Matth talk contribs uploaded File:Timeline.png
- 08:52, 5 July 2019 Matth talk contribs created page File:Antibiotics.png
- 08:52, 5 July 2019 Matth talk contribs uploaded File:Antibiotics.png
- 08:52, 5 July 2019 Matth talk contribs created page Antibiotics (Created page with "The filamentous actinomycetes make around two thirds of all known natural product antibiotics and about half of the antibiotics used clinically.")
- 16:04, 4 July 2019 J Newitt talk contribs created page ISP2 (Created page with "=International Streptomyces Project-2 (ISP-2)= ISP-2 is a rich medium that is routinely used for cultivating Streptmyces lydicus along with various other plant root associate...")
- 15:50, 4 July 2019 Tommclean talk contribs created page YP (Created page with "See YPD")
- 15:42, 4 July 2019 Tommclean talk contribs created page YPD (Created page with "=Yeast Peptone Dextrose= Yeast Peptone Dextrose (YPD) medium is composed of Yeast Peptone medium (YP) with the addition of dextrose (D-glucose). YPD is a general medium used...")
- 15:25, 4 July 2019 Test talk contribs created page Nucleic Acid Methods (Created page with "ChIP Seq")
- 13:07, 4 July 2019 Matth talk contribs created page File:Ps.png
- 13:07, 4 July 2019 Matth talk contribs uploaded File:Ps.png
- 12:59, 4 July 2019 Matth talk contribs created page File:Pseudonocardia.png (Colonies of two different ant-associated Pseudonocardia strains growing on SFM agar)
- 12:59, 4 July 2019 Matth talk contribs uploaded File:Pseudonocardia.png (Colonies of two different ant-associated Pseudonocardia strains growing on SFM agar)
- 12:55, 4 July 2019 Matth talk contribs created page File:Streptomyces.png
- 12:55, 4 July 2019 Matth talk contribs uploaded File:Streptomyces.png
- 12:52, 4 July 2019 Matth talk contribs created page File:Streptomyces.gif
- 12:52, 4 July 2019 Matth talk contribs uploaded File:Streptomyces.gif
- 12:47, 4 July 2019 Matth talk contribs created page Micromonospora (Created page with "In the family <em>[https://en.wikipedia.org/wiki/Micromonosporaceae Micromonosporaceae]</em>, best known for making [https://en.wikipedia.org/wiki/Gentamicin gentamicin] which...")
- 12:40, 4 July 2019 Matth talk contribs created page Salinospora (Created page with "<Salinospora</em> is a genus of marine actinomycetes in the family <em>Micromonosporaceae</em. best known for making [salinosporamide a salinosporamide A] which isn phase 3 cl...")
- 12:38, 4 July 2019 Matth talk contribs created page Actinoplanes (Created page with "In the family <em>[https://en.wikipedia.org/wiki/Micromonosporaceae Micromonosporaceae]</em> and best known for having motile spores.")
- 11:55, 4 July 2019 Matth talk contribs created page Saccharopolyspora (Created page with "In the family Pseudonocardiaceae along with [[<em>Amycolatopsis]]</em> and <em>Pseudonocardia</em>")
- 11:49, 4 July 2019 Matth talk contribs created page Amycolatopsis (Created page with "Circular genomes? Best known species is <em>A. orientalis</em> which makes the glycopeptiode vancomycin, used as a last resort drug against MRSA")
- 17:12, 3 July 2019 Alicia talk contribs created page Cloning and Expression of Gene Clusters (Created page with "TAR cloning Heterologous expression of gene clusters")
- 16:47, 3 July 2019 Alicia talk contribs created page High GC PCR (Created page with "Actinobacteria typically have a high GC content in their DNA. High GC DNA templates can be difficult to amplify by PCR because of stronger hydrogen bonding, and possible forma...")
- 15:55, 3 July 2019 ActinoCommittee talk contribs changed group membership for Alicia from (none) to administrator, interface administrator and bureaucrat
- 15:54, 3 July 2019 User account Alicia talk contribs was created by ActinoCommittee talk contribs
- 11:06, 3 July 2019 ActinoCommittee talk contribs changed group membership for DaveMark from (none) to administrator, interface administrator and bureaucrat
- 11:03, 3 July 2019 User account DaveMark talk contribs was created by ActinoCommittee talk contribs
- 09:21, 3 July 2019 User account Lucasbalis talk contribs was created by Tommclean talk contribs and password was sent by email (Setup)
- 09:12, 3 July 2019 Matth talk contribs created page File:ForBGC.png
- 09:12, 3 July 2019 Matth talk contribs uploaded File:ForBGC.png
- 09:03, 3 July 2019 ActinoCommittee talk contribs changed group membership for Tommclean from (none) to administrator, interface administrator and bureaucrat
- 09:01, 3 July 2019 User account Tommclean talk contribs was created by ActinoCommittee talk contribs (Its my first account)
- 13:27, 2 July 2019 MFeeney talk contribs created page Gause's No. 1 (Created page with "=Gause's No. 1 medium= ==Preparation-- Per litre: *20g soluble starch *1g KNO<sub>3</sub> *0.5g NaCl *0.5g K<sub>2</sub>HPO<sub>4</sub> *0.5g MgSO<sub>4</sub> *0.01g FeSO...")
- 12:01, 2 July 2019 ActinoCommittee talk contribs changed group membership for MFeeney from (none) to administrator
- 11:59, 2 July 2019 User account MFeeney talk contribs was created by ActinoCommittee talk contribs
- 11:04, 2 July 2019 Matth talk contribs created page Plasmids (Created page with "150px|Plasmids|link=Plasmids pSET152 pMS82")
- 10:44, 2 July 2019 Matth talk contribs created page File:Actinobase-icons-trans Protocols.png
- 10:44, 2 July 2019 Matth talk contribs uploaded File:Actinobase-icons-trans Protocols.png
- 10:44, 2 July 2019 Matth talk contribs created page File:Actinobase-icons-trans Organisms.png
- 10:44, 2 July 2019 Matth talk contribs uploaded File:Actinobase-icons-trans Organisms.png
- 10:43, 2 July 2019 Matth talk contribs created page File:Actinobase-icons-trans Plasmids.png
- 10:43, 2 July 2019 Matth talk contribs uploaded File:Actinobase-icons-trans Plasmids.png
- 10:43, 2 July 2019 Matth talk contribs created page File:Actinobase-icons-trans Media-recipes.png
- 10:43, 2 July 2019 Matth talk contribs uploaded File:Actinobase-icons-trans Media-recipes.png
- 10:43, 2 July 2019 Matth talk contribs created page File:Actinobase-icons-trans Background.png
- 10:43, 2 July 2019 Matth talk contribs uploaded File:Actinobase-icons-trans Background.png
- 10:40, 2 July 2019 Matth talk contribs created page File:StrepBible.png
- 10:40, 2 July 2019 Matth talk contribs uploaded File:StrepBible.png
- 16:56, 26 June 2019 User account Laia talk contribs was created by ActinoCommittee talk contribs
- 16:10, 26 June 2019 User account N Som talk contribs was created by Sam Prudence talk contribs
- 11:50, 26 June 2019 ActinoCommittee talk contribs created page MM (Created page with "=MM Medium= ==Preparation== Per litre: *0.5g L-asparagine *0.5g K2HPO4 *0.2g MgSO4*7H2O *0.01g FeSO4*7H2O *10g glucose (added after autoclaving) *10g agar *=1L dH2O '''Instr...")
- 14:58, 25 June 2019 ActinoCommittee talk contribs created page DNA (Created page with "=Difco Nutrient Agar (DNA)= Difco Nutrient Agar is a rich medium commercially available from BD and routinely used for cultivating many non-fastidious organisms. It contains...")
- 15:44, 24 June 2019 User account J Newitt talk contribs was created by Sam Prudence talk contribs
- 20:00, 20 June 2019 Sam Prudence talk contribs created page YEME (Created page with " =YEME Medium= ==For 1 Litre:== *3g Yeast Extract *3g Malt Extract *5g Peptone *10g Glucose *Up to 1L dH2O '''Instructions''' #Add 3g Yeast Extract, 3g Malt Extract, 5g Pept...")
- 19:54, 20 June 2019 Sam Prudence talk contribs created page LB (Created page with "=LB= Known as Lennox Broth or Lysogeny Broth ==For 1 Litre:== *5g Yeast Extract *10g NaCl *10g Tryptone *Up to 1L dH2O '''Instructions''' #Add 5g Yeast Extract, 10g NaCl and...")
- 19:39, 20 June 2019 Sam Prudence talk contribs created page PCR (Created page with "Streptomyces Colony PCR")
- 19:39, 20 June 2019 Sam Prudence talk contribs moved page General ''Streptomyces'' growth and maintenance to General growth and maintenance without leaving a redirect
- 19:38, 20 June 2019 Sam Prudence talk contribs moved page General Streptomyces growth and maintenance to General ''Streptomyces'' growth and maintenance without leaving a redirect
- 19:37, 20 June 2019 Sam Prudence talk contribs created page General Streptomyces growth and maintenance (Created page with "Spore Preparations & Titres Options for Growth in Liquid Medium")
- 19:24, 20 June 2019 Sam Prudence talk contribs created page DNB (Created page with "=Difco Nutrient Broth (DNB)= ==For 1 Litre:== *4g Difco Nutrient Broth Powder *10g Agar *1L dH2O water '''Instructions''' #For solid medium, add 0.8g of DNB powder into each...")
- 15:55, 20 June 2019 Matth talk contribs created page Streptomyces formicae (Created page with "Holmes NA, Devine R, Qin Z, Seipke RF, Wilkinson B, Hutchings MI (2018). Complete genome sequence of Streptomyces formicae KY5, the formicamycin producer. J Biotechnol. 265:11...")
- 15:34, 20 June 2019 Matth talk contribs created page 16S rRNA metaprofiling (Created page with "[https://greengenes.secondgenome.com Green Genes]")