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Combined display of all available logs of ActinoBase. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 16:22, 18 May 2021 H Otani talk contribs created page PTYM19 (Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector...")
- 16:22, 18 May 2021 H Otani talk contribs created page PTOYAMAcos (Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector...")
- 10:01, 26 April 2021 User account S Jarmusch talk contribs was created by Sam Prudence talk contribs
- 11:48, 5 January 2021 User account Parva Zarei talk contribs was created by Sam Prudence talk contribs
- 08:59, 18 August 2020 Alicia talk contribs created page Rhodococcus erythropolis (Created page with "==Organism Page== === ''Rhodococcus erythropolis'' === ===Background=== ===Growth & Maintenance=== GYM =====Sporulation===== Nonsporulating ===Strains=== <em>Rhodo...")
- 12:27, 6 June 2020 Sam Prudence talk contribs created page File:Graphical overview LFM.png
- 12:27, 6 June 2020 Sam Prudence talk contribs uploaded File:Graphical overview LFM.png
- 12:23, 6 June 2020 Sam Prudence talk contribs created page File:LFM plasmid maps.png
- 12:23, 6 June 2020 Sam Prudence talk contribs uploaded File:LFM plasmid maps.png
- 12:07, 6 June 2020 Sam Prudence talk contribs created page Meganuclease I-SceI based system for gene deletions (Created page with "=Gene deletion in actinomycetes using I-SceI meganuclease= This protocol allows the reader to make clean deletions in any actinomycete species using an efficient method to gen...")
- 11:54, 6 June 2020 Sam Prudence talk contribs created page Microbispora (Created page with "=<i>Microbispora</i>= Actinobacteria / Actinobacteria / Streptosporangiales / Streptosporangiaceae / <i>Microbispora</i> <i>Microbispora</i> is a genus of aerobic, Gram-posit...")
- 11:53, 6 June 2020 Sam Prudence talk contribs created page File:Microbispora.png
- 11:53, 6 June 2020 Sam Prudence talk contribs uploaded File:Microbispora.png
- 11:04, 6 May 2020 MFeeney talk contribs created page File:SsDNA recombineering oligos.png (oligonucleotide design for ssDNA recombineering)
- 11:04, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA recombineering oligos.png (oligonucleotide design for ssDNA recombineering)
- 10:16, 6 May 2020 MFeeney talk contribs created page File:SsDNA recombineering protocol.png (figure illustrating two key steps in the ssDNA recombineering protocol)
- 10:16, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA recombineering protocol.png (figure illustrating two key steps in the ssDNA recombineering protocol)
- 10:13, 6 May 2020 MFeeney talk contribs created page File:SsDNA diagnostic pcr.png (description of restriction polymorphism and mismatch PCR approaches to identify the mutant allele generated by ssDNA recombineering)
- 10:13, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA diagnostic pcr.png (description of restriction polymorphism and mismatch PCR approaches to identify the mutant allele generated by ssDNA recombineering)
- 11:07, 5 May 2020 MFeeney talk contribs created page Isolation of phages from environmental samples (Created page with "=<strong>Isolation of phages from environmental samples</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces lividans</em> s...")
- 10:44, 5 May 2020 MFeeney talk contribs created page Isolation of phages from single plaques (Created page with "=<strong>Isolation of phages from single plaques</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</em> with...")
- 10:09, 5 May 2020 MFeeney talk contribs created page High titre preparation of phage (Created page with "=<strong>High titre preparation of ''Streptomyces'' phage</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</e...")
- 09:47, 5 May 2020 MFeeney talk contribs created page File:PhiC31 serial dilution.jpg (Plaque assay: serial dilution of phiC31 on pglW- S. coelicolor)
- 09:47, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 serial dilution.jpg (Plaque assay: serial dilution of phiC31 on pglW- S. coelicolor)
- 09:02, 5 May 2020 MFeeney talk contribs created page Streptomyces Bacteriophage plaque assay (Created page with "=<strong>Streptomyces bacteriophage plaque assay</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</em> with...")
- 08:34, 5 May 2020 MFeeney talk contribs created page File:PhiC31 plaque assay.jpg (PhiC31 plaque assay on a pglW- strain of S. coelicolor)
- 08:34, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 plaque assay.jpg (PhiC31 plaque assay on a pglW- strain of S. coelicolor)
- 08:31, 5 May 2020 MFeeney talk contribs deleted page File:PhiC31 serial dilution.jpg
- 08:31, 5 May 2020 MFeeney talk contribs created page File:PhiC31 serial dilution.jpg (PhiC31 plaque assay)
- 08:31, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 serial dilution.jpg (PhiC31 plaque assay)
- 22:37, 23 April 2020 LisAlgora talk contribs created page Lambda DNA/HindIII Ladder (Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate...")
- 22:35, 23 April 2020 LisAlgora talk contribs created page Lambda DNA/HindIII ladder (Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate...")
- 22:23, 23 April 2020 LisAlgora talk contribs created page Solutions for Southern Blotting (Created page with "=Stock Solutions to Prepare in Advance= {| class="wikitable" ! style="font-family:Arial, Helvetica, sans-serif !important;; background-color:rgb(222, 234, 246); color:rgb(0,...")
- 21:55, 23 April 2020 LisAlgora talk contribs created page Consumables for Southern Blotting (Created page with "Roche provides a <b>DIG DNA Labelling and Detection Kit</b> that contains: blocking reagent, Anti-DIG-AP conjugate, Hexanucleotide Mix, DIG DNA Labelling Mix, Klenow Enzyme an...")
- 09:03, 23 April 2020 MFeeney talk contribs moved page Bacteriophage to Streptomyces Bacteriophages without leaving a redirect
- 08:45, 23 April 2020 MFeeney talk contribs created page Bacteriophage (Created page with "=Streptomyces bacteriophages= Prof Paul A Hoskisson, University of Strathclyde. ==Introduction== Bacteriophages (or phages) are amongst the most abundant biological entities...")
- 12:51, 17 April 2020 Sam Prudence talk contribs changed group membership for MHutchings4 from (none) to administrator, interface administrator and bureaucrat
- 12:49, 17 April 2020 User account MHutchings4 talk contribs was created by Sam Prudence talk contribs
- 15:34, 16 April 2020 DaveMark talk contribs created page The ActinoBase e-Seminar (Created page with "=The ActinoBase e-Seminar Series= The ActinoBase e-Seminar Series is an opportunity for both early-career and established researchers in the Actinobacteria community to share...")
- 00:07, 25 March 2020 H Otani talk contribs created page RNA Sequencing (Created page with "#Extract and purify RNA samples according to RNA extraction from Actinobacteria. Add [https://www.thermofisher.com/order/catalog/product/4456740#/4456740 ERCC RNA Spike-In...")
- 23:43, 24 March 2020 H Otani talk contribs created page Natural Products (Created page with "Cloning, Transformation and Expression of Gene Clusters Metabolite extractions Production of microbial volatile organic compounds")
- 21:55, 24 March 2020 H Otani talk contribs created page Protoplast Transformation (Created page with "#Follow the instruction in Protoplasts Formation, Regeneration and Transformation #Add 3.85 ml P buffer to 1 g PEG sterilised. #Add 200 µl PEG-P buffer mixture and a DNA...")
- 17:27, 10 March 2020 Sam Prudence talk contribs created page File:Logo4.png
- 17:27, 10 March 2020 Sam Prudence talk contribs uploaded File:Logo4.png
- 17:17, 10 March 2020 Sam Prudence talk contribs created page File:ActinobaseLogo1.gif
- 17:17, 10 March 2020 Sam Prudence talk contribs uploaded File:ActinobaseLogo1.gif
- 15:09, 28 February 2020 MFeeney talk contribs created page Indicator compounds (Created page with "=Indicator Compounds= ==5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)== '''Uses''' *Detection of β-galactosidase activity on solid media *Bacterial two-hybrid a...")
- 14:33, 28 February 2020 DaveMark talk contribs created page File:PIJ12738 Map.png (Map of PIJ2738)
- 14:33, 28 February 2020 DaveMark talk contribs uploaded File:PIJ12738 Map.png (Map of PIJ2738)
- 14:06, 28 February 2020 User account Admb talk contribs was created by DaveMark talk contribs