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From ActinoBase
Combined display of all available logs of ActinoBase. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 12:46, 26 October 2021 User account Louisa perry jic talk contribs was created by Jake Newitt talk contribs (new account)
- 12:44, 26 October 2021 User account Louisa perry talk contribs was created by Jake Newitt talk contribs and password was sent by email (new account)
- 11:45, 26 October 2021 User account Louisa Perry talk contribs was created by Jake Newitt talk contribs and password was sent by email (Creating account for colleague)
- 08:03, 21 October 2021 Matt Hutchings talk contribs created page Plant-Microbe interactions (Created page with "In recent years it has become clear that actinomycetes like Streptomyces species are not simply free-living soil bacteria. They also interact with plant roots and can colonise...")
- 07:53, 21 October 2021 Matt Hutchings talk contribs created page Plants (Created page with "150px|Protocols|link=Protocols =Protocols organised by category= ==Bacteriophage== *Streptomyces Bacteriophage plaque a...")
- 16:23, 20 October 2021 Sam Prudence talk contribs changed group membership for Kate Duncan from (none) to administrator, interface administrator and bureaucrat
- 16:21, 20 October 2021 User account Kate Duncan talk contribs was created by Sam Prudence talk contribs
- 16:02, 20 October 2021 Sam Prudence talk contribs changed group membership for Jake Newitt from (none) to administrator, interface administrator and bureaucrat
- 16:01, 20 October 2021 User account Jake Newitt talk contribs was created by Sam Prudence talk contribs
- 14:59, 20 October 2021 User account Sergey Group talk contribs was created by Sam Prudence talk contribs
- 14:57, 20 October 2021 Sam Prudence talk contribs changed group membership for J Newitt from (none) to administrator, interface administrator and bureaucrat
- 14:53, 20 October 2021 Sam Prudence talk contribs changed group membership for Matt Hutchings from (none) to administrator, interface administrator and bureaucrat
- 13:14, 28 July 2021 User account Matt Hutchings talk contribs was created by Sam Prudence talk contribs
- 16:26, 18 May 2021 H Otani talk contribs created page PCRISPR-dCas9 (Created page with "==References== #Tong Y, Charusanti P, Zhang L, Weber T, Lee SY. CRISPR-Cas9 Based Engineering of Actinomycetal Genomes. ''ACS Synth Biol'' 2015 Sep 18;4(9):1020-9. doi: 10.102...")
- 16:25, 18 May 2021 H Otani talk contribs created page PCRISPR-Cas9 (Created page with "==References== #Tong Y, Charusanti P, Zhang L, Weber T, Lee SY. CRISPR-Cas9 Based Engineering of Actinomycetal Genomes. ''ACS Synth Biol'' 2015 Sep 18;4(9):1020-9. doi: 10.102...")
- 16:23, 18 May 2021 H Otani talk contribs created page PTYM18 (Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector...")
- 16:22, 18 May 2021 H Otani talk contribs created page PTYM19 (Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector...")
- 16:22, 18 May 2021 H Otani talk contribs created page PTOYAMAcos (Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector...")
- 10:01, 26 April 2021 User account S Jarmusch talk contribs was created by Sam Prudence talk contribs
- 11:48, 5 January 2021 User account Parva Zarei talk contribs was created by Sam Prudence talk contribs
- 08:59, 18 August 2020 Alicia talk contribs created page Rhodococcus erythropolis (Created page with "==Organism Page== === ''Rhodococcus erythropolis'' === ===Background=== ===Growth & Maintenance=== GYM =====Sporulation===== Nonsporulating ===Strains=== <em>Rhodo...")
- 12:27, 6 June 2020 Sam Prudence talk contribs created page File:Graphical overview LFM.png
- 12:27, 6 June 2020 Sam Prudence talk contribs uploaded File:Graphical overview LFM.png
- 12:23, 6 June 2020 Sam Prudence talk contribs created page File:LFM plasmid maps.png
- 12:23, 6 June 2020 Sam Prudence talk contribs uploaded File:LFM plasmid maps.png
- 12:07, 6 June 2020 Sam Prudence talk contribs created page Meganuclease I-SceI based system for gene deletions (Created page with "=Gene deletion in actinomycetes using I-SceI meganuclease= This protocol allows the reader to make clean deletions in any actinomycete species using an efficient method to gen...")
- 11:54, 6 June 2020 Sam Prudence talk contribs created page Microbispora (Created page with "=<i>Microbispora</i>= Actinobacteria / Actinobacteria / Streptosporangiales / Streptosporangiaceae / <i>Microbispora</i> <i>Microbispora</i> is a genus of aerobic, Gram-posit...")
- 11:53, 6 June 2020 Sam Prudence talk contribs created page File:Microbispora.png
- 11:53, 6 June 2020 Sam Prudence talk contribs uploaded File:Microbispora.png
- 11:04, 6 May 2020 MFeeney talk contribs created page File:SsDNA recombineering oligos.png (oligonucleotide design for ssDNA recombineering)
- 11:04, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA recombineering oligos.png (oligonucleotide design for ssDNA recombineering)
- 10:16, 6 May 2020 MFeeney talk contribs created page File:SsDNA recombineering protocol.png (figure illustrating two key steps in the ssDNA recombineering protocol)
- 10:16, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA recombineering protocol.png (figure illustrating two key steps in the ssDNA recombineering protocol)
- 10:13, 6 May 2020 MFeeney talk contribs created page File:SsDNA diagnostic pcr.png (description of restriction polymorphism and mismatch PCR approaches to identify the mutant allele generated by ssDNA recombineering)
- 10:13, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA diagnostic pcr.png (description of restriction polymorphism and mismatch PCR approaches to identify the mutant allele generated by ssDNA recombineering)
- 11:07, 5 May 2020 MFeeney talk contribs created page Isolation of phages from environmental samples (Created page with "=<strong>Isolation of phages from environmental samples</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces lividans</em> s...")
- 10:44, 5 May 2020 MFeeney talk contribs created page Isolation of phages from single plaques (Created page with "=<strong>Isolation of phages from single plaques</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</em> with...")
- 10:09, 5 May 2020 MFeeney talk contribs created page High titre preparation of phage (Created page with "=<strong>High titre preparation of ''Streptomyces'' phage</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</e...")
- 09:47, 5 May 2020 MFeeney talk contribs created page File:PhiC31 serial dilution.jpg (Plaque assay: serial dilution of phiC31 on pglW- S. coelicolor)
- 09:47, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 serial dilution.jpg (Plaque assay: serial dilution of phiC31 on pglW- S. coelicolor)
- 09:02, 5 May 2020 MFeeney talk contribs created page Streptomyces Bacteriophage plaque assay (Created page with "=<strong>Streptomyces bacteriophage plaque assay</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</em> with...")
- 08:34, 5 May 2020 MFeeney talk contribs created page File:PhiC31 plaque assay.jpg (PhiC31 plaque assay on a pglW- strain of S. coelicolor)
- 08:34, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 plaque assay.jpg (PhiC31 plaque assay on a pglW- strain of S. coelicolor)
- 08:31, 5 May 2020 MFeeney talk contribs deleted page File:PhiC31 serial dilution.jpg
- 08:31, 5 May 2020 MFeeney talk contribs created page File:PhiC31 serial dilution.jpg (PhiC31 plaque assay)
- 08:31, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 serial dilution.jpg (PhiC31 plaque assay)
- 22:37, 23 April 2020 LisAlgora talk contribs created page Lambda DNA/HindIII Ladder (Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate...")
- 22:35, 23 April 2020 LisAlgora talk contribs created page Lambda DNA/HindIII ladder (Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate...")
- 22:23, 23 April 2020 LisAlgora talk contribs created page Solutions for Southern Blotting (Created page with "=Stock Solutions to Prepare in Advance= {| class="wikitable" ! style="font-family:Arial, Helvetica, sans-serif !important;; background-color:rgb(222, 234, 246); color:rgb(0,...")
- 21:55, 23 April 2020 LisAlgora talk contribs created page Consumables for Southern Blotting (Created page with "Roche provides a <b>DIG DNA Labelling and Detection Kit</b> that contains: blocking reagent, Anti-DIG-AP conjugate, Hexanucleotide Mix, DIG DNA Labelling Mix, Klenow Enzyme an...")
- 09:03, 23 April 2020 MFeeney talk contribs moved page Bacteriophage to Streptomyces Bacteriophages without leaving a redirect
- 08:45, 23 April 2020 MFeeney talk contribs created page Bacteriophage (Created page with "=Streptomyces bacteriophages= Prof Paul A Hoskisson, University of Strathclyde. ==Introduction== Bacteriophages (or phages) are amongst the most abundant biological entities...")
- 12:51, 17 April 2020 Sam Prudence talk contribs changed group membership for MHutchings4 from (none) to administrator, interface administrator and bureaucrat
- 12:49, 17 April 2020 User account MHutchings4 talk contribs was created by Sam Prudence talk contribs
- 15:34, 16 April 2020 DaveMark talk contribs created page The ActinoBase e-Seminar (Created page with "=The ActinoBase e-Seminar Series= The ActinoBase e-Seminar Series is an opportunity for both early-career and established researchers in the Actinobacteria community to share...")
- 00:07, 25 March 2020 H Otani talk contribs created page RNA Sequencing (Created page with "#Extract and purify RNA samples according to RNA extraction from Actinobacteria. Add [https://www.thermofisher.com/order/catalog/product/4456740#/4456740 ERCC RNA Spike-In...")
- 23:43, 24 March 2020 H Otani talk contribs created page Natural Products (Created page with "Cloning, Transformation and Expression of Gene Clusters Metabolite extractions Production of microbial volatile organic compounds")
- 21:55, 24 March 2020 H Otani talk contribs created page Protoplast Transformation (Created page with "#Follow the instruction in Protoplasts Formation, Regeneration and Transformation #Add 3.85 ml P buffer to 1 g PEG sterilised. #Add 200 µl PEG-P buffer mixture and a DNA...")
- 17:27, 10 March 2020 Sam Prudence talk contribs created page File:Logo4.png
- 17:27, 10 March 2020 Sam Prudence talk contribs uploaded File:Logo4.png
- 17:17, 10 March 2020 Sam Prudence talk contribs created page File:ActinobaseLogo1.gif
- 17:17, 10 March 2020 Sam Prudence talk contribs uploaded File:ActinobaseLogo1.gif
- 15:09, 28 February 2020 MFeeney talk contribs created page Indicator compounds (Created page with "=Indicator Compounds= ==5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)== '''Uses''' *Detection of β-galactosidase activity on solid media *Bacterial two-hybrid a...")
- 14:33, 28 February 2020 DaveMark talk contribs created page File:PIJ12738 Map.png (Map of PIJ2738)
- 14:33, 28 February 2020 DaveMark talk contribs uploaded File:PIJ12738 Map.png (Map of PIJ2738)
- 14:06, 28 February 2020 User account Admb talk contribs was created by DaveMark talk contribs
- 15:31, 20 February 2020 User account GenomeBrowser talk contribs was created by Sam Prudence talk contribs
- 15:26, 20 February 2020 Sam Prudence talk contribs moved page Salinospora to Salinispora
- 15:22, 20 February 2020 Sam Prudence talk contribs created page Genome browser (Created page with "<h2><strong>ActinoBase Genome Browser</strong></h2> Click the link below for the ActinoBase MORF Genome Browser, with antiSMASH integration. This tool has been kindly provide...")
- 19:50, 16 February 2020 JoshKlein talk contribs created page File:BioArtElvis.PNG (BioArt photo from https://doi.org/10.1371/journal.pbio.1000510.)
- 19:50, 16 February 2020 JoshKlein talk contribs uploaded File:BioArtElvis.PNG (BioArt photo from https://doi.org/10.1371/journal.pbio.1000510.)
- 19:49, 16 February 2020 JoshKlein talk contribs created page File:BioArt.png (A picture of various BioArt plates.)
- 19:49, 16 February 2020 JoshKlein talk contribs uploaded File:BioArt.png (A picture of various BioArt plates.)
- 15:27, 31 January 2020 ElmiraM talk contribs created page Streptacidiphilus (Created page with "=<i>Streptacidiphilus</i>= Streptacidiphilus (etymology: twisted, acid-loving) are one of the three genera belonging to the <i>Streptomycetaceae<i> family, along with <i>Strep...")
- 15:23, 31 January 2020 MFeeney talk contribs created page Lambda-red mediated recombination using ssDNA (Created page with "=Lambda-red mediated recombination using ssDNA= Lambda-red mediated recombination using ssDNA can be used to introduce precise mutations into the genome of <em>Streptomyces</e...")
- 14:47, 31 January 2020 Jordanmurray talk contribs created page Cultivating Actinobacteria for Microscopic Analysis (Created page with "In order to analyse actinobacteria using light microscopy, methods must take into account the multicellular nature of the test organism. Unlike unicellular organisms, actinoba...")
- 16:31, 29 January 2020 R Devine talk contribs created page ChIP Sequencing (Created page with "==General overview== Chromatin Immunoprecipitation and sequencing can be used to characterise protein-DNA interactions. It works by cross-linking proteins to DNA, shearing t...")
- 16:56, 14 November 2019 Sam Prudence talk contribs deleted page Trace elements (content was: "=Trace Elements= Recipe not added", and the only contributor was "Test" (talk))
- 17:29, 7 November 2019 Sam Prudence talk contribs deleted page CRISPR (content before blanking was: "Hutchings Lab protocol for generating CRISPR/Cas Knockouts using pCRISPomyces-2. Written by Rebecca Devine, Matt Hutchings’ Laboratory, University of East Anglia, UK <strong>Overview (see following pages for more deta...")
- 14:19, 25 October 2019 DaveMark talk contribs created page Isolating Actinobacteria from soil environments (Created page with "Isolating Actinobacteria from soil environments =Equipment & Materials= *Sterile containers to transport the soil *A heat block *Plates of Isolation media *A vortexer *¼ Str...")
- 14:08, 25 October 2019 LeightonP talk contribs created page Using pandoc (add page describing use of pandoc to convert Word documents to MediaWiki format)
- 14:01, 25 October 2019 LeightonP talk contribs created page File:Example word file.png (Screenshot of example Word file, before pandoc conversion)
- 14:01, 25 October 2019 LeightonP talk contribs uploaded File:Example word file.png (Screenshot of example Word file, before pandoc conversion)
- 13:17, 25 October 2019 User account CharlieBegley talk contribs was created by DaveMark talk contribs
- 13:16, 25 October 2019 User account MegJeppesen talk contribs was created by DaveMark talk contribs (New Member)
- 13:15, 25 October 2019 User account MollyKeithBaker talk contribs was created by DaveMark talk contribs (New Member)
- 13:14, 25 October 2019 User account ElmiraM talk contribs was created by DaveMark talk contribs
- 15:16, 17 October 2019 Alicia talk contribs created page Site-specific phage recombinase (Created page with "<strong>Integration sites</strong> You can check the sequence of your organism to see if it contains the appropriate integration site for your chosen plasmid: *ΦBT1, attB...")
- 15:44, 10 October 2019 J Newitt talk contribs created page Links master page (Created page with "=Useful Links= ==Bioinformatics== ===16S rRNA metaprofiling=== [https://greengenes.secondgenome.com Green Genes] 16S rRNA gene database [https://www.arb-silva.de/ SILVA]...")
- 16:25, 30 September 2019 DaveMark talk contribs created page Aminoglycosides (Created page with "=Intro= The aminoglycoside antibiotics are a class of amino containing oligosaccharides, and are notable for their broad-spectrum bacteriocidal activity - including their abil...")
- 14:35, 27 September 2019 LisAlgora talk contribs created page Southern Blotting (Created page with "There are many different protocols for Southern blotting. This approach uses vacuum transfer pf DNA to a nylon membrane, DIG-labelling of the probe and detection by chromogeni...")
- 13:53, 27 September 2019 EmilyAddington talk contribs created page Actinomycetes Morphology and Lifecycle (Created blank page)
- 08:57, 27 September 2019 User account JoshKlein talk contribs was created by MFeeney talk contribs and password was sent by email
- 08:56, 27 September 2019 User account LouCharkoudian talk contribs was created by MFeeney talk contribs and password was sent by email
- 16:01, 26 September 2019 J Newitt talk contribs created page General Growth and Maintenance of Streptomyces sp. (Created page with "=General growth and maintenance of ''Streptomyces'' species= ''Streptomyces'' are multi-nuclear filamentous organisms that undergo several different stages of growth. This se...")
- 15:25, 26 September 2019 Tommclean talk contribs created page Luria (Created page with "=Luria Broth= Known as Luria Broth (or sometimes, mistakenly, as LB or Lennox medium), this rich medium is used routinely for growth of <em>Escherichia coli</em>. There are se...")
- 15:25, 26 September 2019 Sam Prudence talk contribs created page File:SCOR2.jpg
- 15:25, 26 September 2019 Sam Prudence talk contribs uploaded File:SCOR2.jpg
- 15:23, 26 September 2019 Tommclean talk contribs created page Lennox (Created page with "=Lennox Broth= Known as Lennox Broth (or sometimes, mistakenly, as Luria-Bertani medium), this rich medium is used routinely for growth of <em>Escherichia coli</em>. There are...")
- 15:12, 26 September 2019 Tommclean talk contribs created page R5 (Created page with "=R5= ==Preparation== Make up the following solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *10.12 g MgCl<sub>2</sub>.6H<sub>2</sub>O *10 g Glucose *0.1 g Difco...")