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• 10:41, 13 January 2025 Matt Hutchings talk contribs blocked Test talk contribs with an expiry time of indefinite (account creation disabled)
• 10:40, 13 January 2025 Matt Hutchings talk contribs blocked Louisa perry jic talk contribs with an expiry time of indefinite (account creation disabled)
• 10:39, 13 January 2025 Matt Hutchings talk contribs blocked Damien Gayrard talk contribs with an expiry time of indefinite (account creation disabled)
• 10:34, 13 January 2025 User account Gageowens talk contribs was created by Matt Hutchings talk contribs (New editor)
• 09:41, 29 November 2024 Matt Hutchings talk contribs created page File:PIJ86.jpg
• 09:41, 29 November 2024 Matt Hutchings talk contribs uploaded File:PIJ86.jpg
• 10:15, 28 November 2024 Matt Hutchings talk contribs created page PIJ86 (Created page with "Plasmid sequence of pIJ86 is [here https://www.ncbi.nlm.nih.gov/nuccore/2831519705]")
• 11:07, 14 November 2024 Matt Hutchings talk contribs created page Bioinformatics Ressources (Created page with "===2024 SSAMM - Bioinformatics Tools and Online Resources=== ====Strains==== [http://streptomyces.org.uk Streptomyces.org] Links to resources including StrepDB and a free download of the Streptomyces manual. [https://bacdive.dsmz.de/ BacDive (The Bacterial Diversity Metadatabase) ] The world’s largest database for standardized bacterial information. Look up your strain, its morphology, culture and growth condition, DNA sequence accessions, chemotaxonomic features et...")
• 10:36, 14 November 2024 Matt Hutchings talk contribs created page Useful resources can be found here (Created page with "===2024 SSAMM - Bioinformatics Tools and Online Resources===")
• 10:33, 14 November 2024 Matt Hutchings talk contribs created page JIC Summer School in Applied Molecular Microbiology (Created page with "=== JIC Summer School in Applied Molecular Microbiology === Bionformatics Resources This summer school is organised by the John Innes Centre and is held every two years in Dubrovnik, hosted by the [https://iuc.hr/ IUC Dubrovnik]. Typically we host around 50 students (typically PhD students and postdocs) and the course runs over eight days. Info on the 2024 summer school can be found [https://www.jic.ac.uk/training-careers/summ...")
• 10:01, 14 November 2024 Matt Hutchings talk contribs created page JIC Summer School in Applied Microbiology (Created page with "JIC Summer School in Applied Molecular Microbiology This summer school is organised by the John Innes Centre and is held every two years in Dubrovnik, hosted by the [https://iuc.hr/] IUC Dubrovnik. Typically we host around 50 students (typically PhD students and postdocs) an the course runs over eight days. Info on the 2024 summer school can be found [https://www.jic.ac.uk/training-careers/summer-schools/applied-molecular-microbiology/ here]")
• 09:06, 31 July 2024 Matt Hutchings talk contribs created page PIJ6021 (Created page with "Plasmid pIJ6021 allows for high level protein production in ''Streptomyces'' species. This plasmid was first described by [https://www.sciencedirect.com/science/article/pii/0378111995005452?via%3Dihub Takano et al 1995] and utilises the pIJ101 origin of replication which leads to high copy number, sometimes approaching 100 copies per cell. It includes the thiostrepton inducible ''tipA'' promoter to induce expression of the target gene.")
• 08:55, 31 July 2024 Matt Hutchings talk contribs created page PUZ8002 (Created page with "This is a helper plasmid that encodes tha ,achnery required for conjugating other plasmids into 'Streptomyces'. For everything you ever needed to know about pUZ8002 please read this paper: Larcombe et al, 2024. Sequence and origin of the Streptomyces intergenetic-conjugation helper plasmid pUZ8002. Access Micro 6:000808.v3. https://doi.org/10.1099/acmi.0.000808.v3")
• 12:43, 18 July 2024 Matt Hutchings talk contribs created page Protein production in Streptomyces (Created page with """Protocol for "Streptomyces" protein expression"" ""Background"" Plasmid pIJ6021 allows for high level protein production in "Streptomyces" species. This plasmid was first described by Bibb et al in 19951 and utilises the pIJ101 origin of replication which leads to high copy number, sometimes approaching 100 copies per cell. It includes the thiostrepton inducible tipA promoter to induce expression of the target gene. However, pIJ6021 lacks an E. coli replicon, meanin...")
• 09:27, 7 June 2024 Matt Hutchings talk contribs created page Streptomyces lividans (Created page with "== ''Streptomyces lividans'' 66(2) == ===Background=== <em>Streptomyces lividans</em> 66 (also known as 1326) is a model organism for <em>Streptomyces</em> research and particularly useful for heterologous production of proteins and small molecules since it does not restrict foreign methylated DNA.Thew most commonly used strais are TK23 and TK24 which are prototrophic and plasmid free. S. lividans strains 66 and TK24 have both been genome sequenced. ===Growth & Mai...")
• 12:00, 12 March 2024 User account Annie talk contribs was created by Matt Hutchings talk contribs (New editor)
• 21:09, 22 January 2024 Gilles talk contribs created page CUBIC (Created page with "=CUBIC= CUBC is a cumate-based inducible CRISPR interference (CRISPRi) system that allows efficient and inducible gene knockdown in Streptomyces. Click the link to find out more: CUBIC: A Versatile Cumate-Based Inducible CRISPRi System in Streptomyces pCB-1 PCB-2 ==Organisms== CUBIC has been confirmed to work for the following species: *<em>Streptomyces coelicolor</em> *<em>Streptomyces lividans</em> *<em>Streptomyces venezuelae</em> *<em>Streptomyc...")
• 16:44, 1 November 2023 RTEvans talk contribs moved page RRNA depletion with Streptomyces coelicolor A3(2) specific probes. to RRNA depletion with Streptomyces coelicolor A3(2) specific probes over redirect
• 16:44, 1 November 2023 RTEvans talk contribs deleted redirect RRNA depletion with Streptomyces coelicolor A3(2) specific probes by overwriting (Deleted to make way for move from "RRNA depletion with Streptomyces coelicolor A3(2) specific probes.")
• 16:42, 1 November 2023 RTEvans talk contribs moved page RRNA depletion with Streptomyces coelicolor A3(2) specific probes to RRNA depletion with Streptomyces coelicolor A3(2) specific probes. (Removing capitalised R in rRNA)
• 15:59, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelD.png (larger)
• 15:59, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelC.png (larger)
• 15:58, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelA.png (larger)
• 15:56, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelD.png (Larger)
• 15:52, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelB.png (Larger)
• 15:51, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelA.png (Larger)
• 15:49, 1 November 2023 RTEvans talk contribs uploaded a new version of File:PanelA.png (Larger)
• 15:36, 1 November 2023 RTEvans talk contribs created page File:PanelD.png (Genome mapping of S. coelicolor samples following rRNA depletion using protocol described on this page)
• 15:36, 1 November 2023 RTEvans talk contribs uploaded File:PanelD.png (Genome mapping of S. coelicolor samples following rRNA depletion using protocol described on this page)
• 15:35, 1 November 2023 RTEvans talk contribs created page File:PanelC.png (Genome mapping of S. coelicolor samples following rRNA depletion using Illumina Ribo-zero rRNA Removal Kit (Bacteria))
• 15:35, 1 November 2023 RTEvans talk contribs uploaded File:PanelC.png (Genome mapping of S. coelicolor samples following rRNA depletion using Illumina Ribo-zero rRNA Removal Kit (Bacteria))
• 15:35, 1 November 2023 RTEvans talk contribs created page File:PanelB.png (Genome mapping of S. coelicolor samples following rRNA depletion using NEBNext rRNA Depletion Kit (Bacteria))
• 15:35, 1 November 2023 RTEvans talk contribs uploaded File:PanelB.png (Genome mapping of S. coelicolor samples following rRNA depletion using NEBNext rRNA Depletion Kit (Bacteria))
• 15:33, 1 November 2023 RTEvans talk contribs created page File:PanelA.png (Genome mapping of S. coelicolor samples following rRNA depletion with Qiagen FastSelect 5S/16S/23S kit)
• 15:33, 1 November 2023 RTEvans talk contribs uploaded File:PanelA.png (Genome mapping of S. coelicolor samples following rRNA depletion with Qiagen FastSelect 5S/16S/23S kit)
• 15:26, 1 November 2023 RTEvans talk contribs created page File:RRNA depletion table 1.png (Table 1 - list of abundant non mRNA RNAs in S. coelicolor A3(2))
• 15:26, 1 November 2023 RTEvans talk contribs uploaded File:RRNA depletion table 1.png (Table 1 - list of abundant non mRNA RNAs in S. coelicolor A3(2))
• 15:25, 1 November 2023 RTEvans talk contribs created page RRNA depletion with Streptomyces coelicolor A3(2) specific probes (Created page with "==Organisms== This protocol has been tested with <em>Streptomyces coelicolor</em> A3(2) MT1110 ==rRNA depletion with <em>Streptomyces coelicolor</em> A3(2) specific probes== rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depleti...")
• 11:26, 1 November 2023 User account RTEvans talk contribs was created by MFeeney talk contribs (new user account)
• 07:36, 11 October 2023 User account Gilles talk contribs was created by Matt Hutchings talk contribs
• 17:01, 28 September 2023 User account Mgonza local talk contribs was created automatically
• 16:16, 12 September 2023 Matt Hutchings talk contribs undeleted page CRISPR (5 revisions)
• 15:59, 12 September 2023 Matt Hutchings talk contribs created page MtrA methods are here (Created page with "Som N, Devine R, Chandra G, Martins C, Wilkinson B and Hutchings MI (2023). MtrA regulates the expression of developmental genes, including BldM and WhiI, in Streptomyces vene...")
• 15:57, 12 September 2023 Matt Hutchings talk contribs created page Methods (Created page with "This allows you to publish supplementary methods to support, and cite, in your publications. Email matt.hutchings@jic.ac.uk Som N, Devine R, Chandra G, Martins C, Wilkinson...")
• 07:52, 21 August 2023 Matt Hutchings talk contribs created page VProtocols (Created page with "Below are links to video protocols created by our members: Streptomyces spore preparation by Lorena Fernández-Martínez <Fernandl@edgehill.ac.uk>")
• 09:18, 30 June 2023 User account Tomgreg talk contribs was created by Matt Hutchings talk contribs
• 07:45, 30 June 2023 User account Thomgregory talk contribs was created by Matt Hutchings talk contribs
• 08:18, 29 June 2023 User account Thomgreg talk contribs was created by Matt Hutchings talk contribs and password was sent by email (New editor)
• 13:41, 13 September 2022 User account LabInsubria talk contribs was created by MFeeney talk contribs
• 10:01, 28 July 2022 User account Nmvior talk contribs was created by MFeeney talk contribs