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Combined display of all available logs of ActinoBase. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 20:14, 2 August 2019 HeeeenaJ talk contribs created page PIJ12742 (Background)
- 12:45, 2 August 2019 Tommclean talk contribs created page Recruitment (Created page with "=Recruitment= Please feel free to post information and links to job opportunities etc below: *Please also remember to remove the links once filled.")
- 12:03, 2 August 2019 Matth talk contribs created page Groups (Created page with "Alphabetical list of research groups working on Streptomyces and closely related bacteria:")
- 10:35, 2 August 2019 User account R Devine talk contribs was created by Sam Prudence talk contribs
- 08:53, 2 August 2019 Sam Prudence talk contribs moved page Cloning and Expression of Gene Clusters to Cloning, Transformation and Expression of Gene Clusters
- 18:05, 1 August 2019 Sam Prudence talk contribs created page Tri-parental mating (Created page with "=<strong>Tri-parental Mating</strong>= ===Why Tri-Parental Mating?=== Tri-parental mating is useful for conjugating plasmids containing a kanamycin resistane gene as a selecta...")
- 15:57, 1 August 2019 Tommclean talk contribs created page SMMS (Created page with "=SMMS (Supplemented liquid minimal media, solid)= SMM is a solid supplemented minimal medium suitable for growth of many ''Streptomyces'' sp. ==Preparation== Per litre: *81.9...")
- 15:56, 1 August 2019 Jacob Hamilton talk contribs created page SPY (Created page with "=Salt Peptone Yeast Agar (SPY)= Salt Peptone Yeast agar is a metal-rich media used to stimulate the activation of cryptic biosynthetic gene clusters as part of antibiotic dis...")
- 15:55, 1 August 2019 Lucasbalis talk contribs created page Protein Methods (Created page with "Codon Optimising Sequences for Heterologous Expression in Streptomyces")
- 15:38, 1 August 2019 Jacob Hamilton talk contribs moved page Potato Dextrose Agar PDA to PDA (Rename)
- 15:36, 1 August 2019 Jacob Hamilton talk contribs moved page PGA to Potato Dextrose Agar PDA (Renaming from glucose to dextrose to maintain consistency)
- 15:33, 1 August 2019 Jacob Hamilton talk contribs created page PGA (Created page with "=Potato Dextrose Agar (PDA)= Potato Dextrose Agar is primarly used for the growth of the opportunistic fungal pathogen ''Scedosporium prolificans''. Thus, it is mostly used...")
- 15:30, 1 August 2019 Tommclean talk contribs created page SMM (Created page with "=SMM (Supplemented liquid minimal media)= Strauch et al., 1991<sup>1</sup> ==Preparation== Per litre: *81.9 mL PEG 6000 (BDH 6.1% w/v in distilled water) *2.5 mL Mg<sub>2</su...")
- 15:24, 1 August 2019 MFeeney talk contribs created page Overview of Streptomyces vectors (Created page with "150px|Plasmids|link=Plasmids =Commonly Used Streptomyces Vectors= This page describes the characteristics of some of the most co...")
- 15:21, 1 August 2019 Alicia talk contribs created page Streptomyces coelicolor A3(2) (Created page with "==Organism Page== === ''Streptomyces coelicolor A3(2)'' === ===Background=== ===Growth & Maintenance=== =====Sporulation===== *Sporulation can be achieved after 3-5 days gr...")
- 11:00, 1 August 2019 MFeeney talk contribs created page HT (Created page with "=Hickey-Tresner agar (HT)= This medium was developed by Hickey and Tresner<sup>[1]</sup> for the growth of some ''Streptomyces'' sp. that did not sporulate well on Bennett's...")
- 13:39, 31 July 2019 MFeeney talk contribs created page Antibiotic stocks and working concentrations (Created page with "{| class="wikitable" ! style="font-weight:bold; background-color:#c0c0c0;" | Antibiotic ! style="font-weight:bold; background-color:#c0c0c0;" | Stock [mg/ml] ! style="font-wei...")
- 13:29, 31 July 2019 User account Paulhoskisson talk contribs was created by MFeeney talk contribs and password was sent by email
- 11:06, 31 July 2019 MFeeney talk contribs created page CM (Created page with "=CM (Complete Medium)= ==Preparation== Per litre: *5g K<sub>2</sub>HPO<sub>4</sub> *0.5g NaCl *0.5g MgSO<sub>4</sub>*7H<sub>2</sub>O *2g Difco Bacto-peptone *1.5g Difco Casa...")
- 08:21, 30 July 2019 MFeeney talk contribs created page IMA (Created page with "=IMA(Instant Mash Agar)= IMA is sometimes also called [https://en.wikipedia.org/wiki/Smash_(instant_mashed_potato) Smash] (after the brand name of the instant potato commonly...")
- 20:47, 29 July 2019 User account HeeeenaJ talk contribs was created by MFeeney talk contribs and password was sent by email
- 10:59, 29 July 2019 CraigAllan talk contribs created page Streptomyces griseus (Created page with "==Organism Page== === ''Streptomyces griseus'' === ===Background=== ===Growth & Maintenance=== =====Sporulation===== ===Strains=== ====Superhosts==== ===Links=== =====Ge...")
- 10:47, 29 July 2019 ActinoCommittee talk contribs changed group membership for CraigAllan from administrator to administrator, interface administrator and bureaucrat
- 10:46, 29 July 2019 ActinoCommittee talk contribs changed group membership for CraigAllan from (none) to administrator
- 10:46, 29 July 2019 User account CraigAllan talk contribs was created by ActinoCommittee talk contribs (Actino Committee)
- 10:07, 29 July 2019 MFeeney talk contribs created page NMMP (Created page with "=Minimal liquid medium (NMMP)= NMMP medium is used to obtain dispersed growth of ''Streptomyces'' sp. with specific carbon sources. ==Preparation== Make up the following sol...")
- 15:12, 28 July 2019 DaveMark talk contribs created page ISP7 (Created page with "=ISP 7= ==Preparation== *15 g Glycerol *0.5 g L-tyrosine *1 g L-asparagine *0.5 g K<sub>2</sub>HPO<sub>4</sub> *0.5 g MgSO<sub>4</sub> x 7H<sub>2</sub>O *0.5 g NaCl *0.01 g...")
- 13:24, 28 July 2019 DaveMark talk contribs created page ISP6 (Created page with "=ISP6= ==Preparation== *36 g Bacto-Peptone Iron Agar (Difco) **15 g Bacto-Peptone **5 g Proteose-peptone ** 0.5 g Ferric ammonium citrate ** 1g K<sub>2</sub>2HPO<sub>4</sub...")
- 12:28, 28 July 2019 DaveMark talk contribs created page File:ISP 6 well.png (6-well plate with ISPs 2-7)
- 12:28, 28 July 2019 DaveMark talk contribs uploaded File:ISP 6 well.png (6-well plate with ISPs 2-7)
- 17:16, 26 July 2019 LisAlgora talk contribs created page R2YE (Created page with "=R2YE= R2YE medium is a variation of R2 containing yeast extract. ==Preparation== Make up the following solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *10.12 g...")
- 16:01, 26 July 2019 LisAlgora talk contribs created page R2 (Created page with "=R2= ==Preparation== #Make up the following solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *10.12 g MgCl<sub>2</sub>.6H<sub>2</sub>O *10 g Glucose *0.1 g Difc...")
- 15:57, 26 July 2019 Laia talk contribs moved page Category:Actinobacteria isolation from marine environments to Actinobacteria isolation from marine environments over redirect
- 15:57, 26 July 2019 Laia talk contribs deleted redirect Actinobacteria isolation from marine environments by overwriting (Deleted to make way for move from "Category:Actinobacteria isolation from marine environments")
- 15:55, 26 July 2019 Laia talk contribs moved page Actinobacteria isolation from marine environments to Category:Actinobacteria isolation from marine environments
- 15:52, 26 July 2019 MFeeney talk contribs created page PUT18C (Created page with "{{DISPLAYTITLE: pUT18C}} =pUT18C= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:51, 26 July 2019 MFeeney talk contribs created page PUT18 (Created page with "{{DISPLAYTITLE: pUT18}} =pUT18= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:51, 26 July 2019 MFeeney talk contribs created page PKT25 (Created page with "{{DISPLAYTITLE: pKT25}} =pKT25= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:50, 26 July 2019 MFeeney talk contribs created page PKNT25 (Created page with "{{DISPLAYTITLE: pKNT25}} =pKNT25= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:50, 26 July 2019 Jordanmurray talk contribs created page Media (Redirected page to Media Recipes) Tag: New redirect
- 15:50, 26 July 2019 MFeeney talk contribs created page PET28a (Created page with "{{DISPLAYTITLE: pET28a}} =pET28a= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:49, 26 July 2019 MFeeney talk contribs created page PIJ773 (Created page with "{{DISPLAYTITLE: pIJ773}} =pIJ773= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:49, 26 July 2019 MFeeney talk contribs created page PSS170 (Created page with "{{DISPLAYTITLE: pSS170}} =pSS170= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:48, 26 July 2019 MFeeney talk contribs created page PRT802 (Created page with "{{DISPLAYTITLE: pRT802}} =pRT802= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:47, 26 July 2019 MFeeney talk contribs created page PMF96 (Created page with "{{DISPLAYTITLE: pMF96}} =pMF96= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:47, 26 July 2019 MFeeney talk contribs created page PKC1132 (Created page with "{{DISPLAYTITLE: pKC1132}} =pKC1132= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:46, 26 July 2019 MFeeney talk contribs created page PIJ12738 (Created page with "{{DISPLAYTITLE: pIJ12738}} =pIJ12738= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:45, 26 July 2019 MFeeney talk contribs created page PIJ8660 (Created page with "{{DISPLAYTITLE: {{DISPLAYTITLE: pIJ10257}} =pIJ8660= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:43, 26 July 2019 ActinoCommittee talk contribs created page Metabolite extractions (Protocols to extract metabolites from Actinobacteria)
- 15:38, 26 July 2019 MFeeney talk contribs created page PIJ733 (Created page with "{{DISPLAYTITLE: pIJ773}} =pIJ773= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:37, 26 July 2019 DaveMark talk contribs created page ISP5 (Created page with "=ISP 5= ==Preparation== *1 g L-asparagine (anhydrous) *10 g Glycerol *1 g K<sub>2</sub>HPO<sub>4</sub> *1 L dH<sub>2</sub>O *1 mL Trace salts solution **0.1 g FeSO<sub>4</s...")
- 15:27, 26 July 2019 REMcHugh talk contribs created page File:Sgoldwiki.png
- 15:27, 26 July 2019 REMcHugh talk contribs uploaded File:Sgoldwiki.png
- 15:22, 26 July 2019 DaveMark talk contribs created page ISP4 (Created page with "=ISP 4= International <i>Streptomyces</i> Project medium 4, also named inorganic salts-starch agar ==Preparation== *10 g Soluble Starch *1 g MgSO<sub>4</sub> x 7H<sub>2</sub>...")
- 15:19, 26 July 2019 REMcHugh talk contribs created page File:Sgoldiniensis.png
- 15:19, 26 July 2019 REMcHugh talk contribs uploaded File:Sgoldiniensis.png
- 15:15, 26 July 2019 MFeeney talk contribs created page PIJ10257 (Created page with "{{DISPLAYTITLE: pIJ10257}} =pIJ10257= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:11, 26 July 2019 REMcHugh talk contribs created page File:Streptomyces goldiniensis.png
- 15:11, 26 July 2019 REMcHugh talk contribs uploaded File:Streptomyces goldiniensis.png
- 15:09, 26 July 2019 ASBirke talk contribs created page MC agar (Created page with "=MC agar= This media is used to screen Streptomyces strains for extracellular protease production and the resulting degradation of the milk powder components on the plate, wh...")
- 15:09, 26 July 2019 Jordanmurray talk contribs created page Staining of ''Streptomyces'' spp. Cell Wall and Nucleic Acids (Redirected page to Staining of Streptomyces spp. Cell Wall and Nucleic Acids) Tag: New redirect
- 15:09, 26 July 2019 User account LRooney talk contribs was created by MFeeney talk contribs (Additional user)
- 15:04, 26 July 2019 MFeeney talk contribs created page Supercos1 (Created page with " =SuperCos1= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 14:59, 26 July 2019 MFeeney talk contribs created page PESAC13 (Created page with "=pESAC13= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 14:52, 26 July 2019 MFeeney talk contribs created page SC (Created page with "=Starch Casein Agar= ==Preparation== Per Litre: *10 g soluble starch *2g K<sub>2</sub>HPO<sub>4</sub> *2g KNO<sub>3</sub> *0.3g casein *0.05g MgSO<sub>4</sub>*7H<sub>2</sub>O...")
- 14:46, 26 July 2019 LisAlgora talk contribs created page SNA (Created page with "=SNA= Soft Nutrient Agar (SNA) ==Preparation== Per Litre: *4g Difco Nutrient Broth Powder *5g Agar *1L dH<sub>2</sub>O '''Instructions''' #Add 0.8g of DNB powder into eac...")
- 14:41, 26 July 2019 MFeeney talk contribs created page OT (Created page with "=OT(oligotrophic agar)= Oligotrophic agar is an extremely nutritionally-poor medium useful for isolating slow-growing actinomyces e.g. from soil. ==Preparation== Per litre:...")
- 14:36, 26 July 2019 REMcHugh talk contribs created page Streptomyces goldiniensis (Created page with "=<em>Streptomyces goldiniensis </em>= <em>Streptomyces goldiniensis </em>is a Gram-positive soil dwelling bacterium that was first isolated from the island of Bermuda in 1969...")
- 14:27, 26 July 2019 MFeeney talk contribs created page HV (Created page with "=HV= ==Preparation== Per Litre: *1g humic acid *1.7g KCl *0.5g Na<sub>2</sub>HPO<sub>4</sub> *0.5g MgSO<sub>4</sub> *0.02g CaCO<sub>3</sub> *0.01g FeSO<sub>4</sub> *1mL VB...")
- 14:24, 26 July 2019 LisAlgora talk contribs created page 2X YT (Created page with "=2x YT= ==Preparation== Per litre: *16 g Tryptone *10 g Yeast Extract *5 g NaCl *1 L dH<sub>2</sub>O '''Instructions''' #Dissolve constituents in 1L dH<sub>2</sub>O #Ste...")
- 14:18, 26 July 2019 DaveMark talk contribs created page ISP3 (Created page with "=ISP 3= International Streptomyces Project media 3 (ISP 3) is a rich general use media. Alternatively called oatmeal agar. ==Preparation== *20 g White Oats *18 g Agar *1 mL...")
- 14:18, 26 July 2019 Jordanmurray talk contribs created page Staining of Streptomyces spp. Cell Wall and Nucleic Acids (Created page with "The following staining technique is a useful method for visualising the actively growing regions of the cell wall, along with the nucleic acids within the cell. The method was...")
- 14:08, 26 July 2019 ActinoCommittee talk contribs created page Emerson medium (Created page with "=Emerson medium= Emerson medium is a rich medium formulated by Emerson et al (1) to isolate mould and Actinobacteria. It is composed of beef extract, yeast extract, peptic di...")
- 13:54, 26 July 2019 User account Jacob Hamilton talk contribs was created by Sam Prudence talk contribs
- 13:51, 26 July 2019 User account ASBirke talk contribs was created by MFeeney talk contribs
- 13:48, 26 July 2019 DaveMark talk contribs created page GYM (Created page with "=Glucose Yeast-Extract Malt-Extract Media= ==Preparation== Per litre: *4 g Glucose *4 g Yeast Extract *10 g Malt Extract *CaCo<sub>3</sub> *12 g Agar *1 L dH<sub>2</sub>O...")
- 13:47, 26 July 2019 User account REMcHugh talk contribs was created by MFeeney talk contribs
- 13:36, 26 July 2019 ActinoCommittee talk contribs moved page Environmental isolation to Actinobacteria isolation from marine environments
- 13:31, 26 July 2019 Jordanmurray talk contribs created page Microscopy (Created page with "=Microscopy of Actinobacteria= ==<em>Streptomyces</em>==")
- 13:25, 26 July 2019 User account RMcHugh talk contribs was created by MFeeney talk contribs
- 13:21, 26 July 2019 ActinoCommittee talk contribs created page Environmental isolation (Protocols to isolate Actinobacteria from different environments)
- 13:18, 26 July 2019 User account LisAlgora talk contribs was created by MFeeney talk contribs
- 13:17, 26 July 2019 User account Jordanmurray talk contribs was created by MFeeney talk contribs
- 13:00, 26 July 2019 User account MunnJ90 talk contribs was created by MFeeney talk contribs
- 11:48, 26 July 2019 MFeeney talk contribs created page Teaching and learning (Created page with "This is the hub for #ActinoBase teaching and learning resources: educational content, outreach, and more! We welcome contributions from the community, so please Contact us...")
- 16:06, 25 July 2019 User account Yvonne Mast talk contribs was created by Sam Prudence talk contribs
- 11:15, 24 July 2019 Alicia talk contribs created page Heterologous expression of gene clusters (Created page with "Gene clusters that have been cloned from their native host can be expressed heterologously in a different host organism. This can help improve production of known products, or...")
- 10:50, 24 July 2019 Alicia talk contribs created page PCAP03 (Created page with " The [https://pubs.acs.org/doi/10.1021/acschembio.5b00658 pCAP03 paper]")
- 08:51, 22 July 2019 Matth talk contribs created page CRISPR (Created page with "Hutchings Lab protocol for generating CRISPR/Cas Knockouts using pCRISPomyces-2. Written by Rebecca Devine, Matt Hutchings’ Laboratory, University of East Anglia, UK <em>O...")
- 20:10, 18 July 2019 Matth talk contribs created page CRISPR/Cas9-mediated genome editing (Created page with "pCRISPomyces2")
- 19:43, 17 July 2019 Matth talk contribs created page PSET152 (Created page with "File:pSET152.png")
- 19:43, 17 July 2019 Matth talk contribs created page File:PSET152.png
- 19:43, 17 July 2019 Matth talk contribs uploaded File:PSET152.png
- 19:40, 17 July 2019 Matth talk contribs created page File:PMS82.png
- 19:40, 17 July 2019 Matth talk contribs uploaded File:PMS82.png
- 19:39, 17 July 2019 Matth talk contribs created page PMS82 (Created page with "Integrating vector derived from the φBT1 attP-int locus and carrying the hygromycin resistance cassette. Described [https://jb.asm.org/content/185/17/5320.long here]")
- 19:27, 17 July 2019 Matth talk contribs created page PCRISPomyces2 (Created page with "file:CRISPR.png")
- 19:27, 17 July 2019 Matth talk contribs created page File:CRISPR.png
- 19:27, 17 July 2019 Matth talk contribs uploaded File:CRISPR.png
- 09:35, 5 July 2019 Matth talk contribs deleted page File:Timeline.png (Deleted old revision 20190705093521!Timeline.png)