Media recipes: Difference between revisions

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[[DNB]] (Difco Nutrient Broth)
{{DISPLAYTITLE:Media Recipes}}


[[LB]] (Lysogeny Broth - sometimes also called Luria Bertani)
[[File:Actinobase-icons-trans_Media-recipes.png|150px|Media Recipes|link=Media_recipes]]


[[MYM]] (Maltose + Yeast Extract + Malt Extract)
=Media Recipes=


[[SFM]] (Soya Flour + Mannitol agar)
Please note that 15 or 20g/L (1.5-2%) agar is added to make solid medium


[[TSB]] (Tryptic Soy Broth)
[[ABB13]]
 
[[A1]]
 
[[CM]] (Complete medium)
 
[[DNA]] (Difco Nutrient Agar) | [[DNB]] (Difco Nutrient Broth) | [[SNA]] (Soft Nutrient Agar)
 
[[Emerson medium]]
 
[[Gause's No. 1]]
 
[[GYM]] (Glucose + Yeast Extract + Malt Extract)
 
[[HT]] (Hickey-Tresner)
 
[[HV]]
 
[[IMA]] (Smash)
 
[[LB]] (Lysogeny Broth) | [[Lennox]] (Lennox agar) | [[Luria]] (Luria agar)
 
[[MC agar]] (Milk Casein Agar)
 
[[MM]] (Minimal medium)
 
[[SFM | MS]] (Mannitol Soya agar - a.k.a. Soya Flour Mannitol (SFM))
 
[[1/2 MS0 | 1/2 MS-S]]
 
[[1/2 MS+B5]]
 
[[MYM]] (Maltose + Yeast Extract + Malt Extract)
 
[[NMMP]] (minimal liquid medium)
 
[[OT]] (oligotrophic agar)
 
[[PDA]] (Potato Dextrose Agar)
 
[[R2]] | [[R2YE]] | [[R5]]
 
[[SC]] (Starch Casein agar)
 
[[SFM]] (Soya Flour Mannitol - a.k.a. Mannitol Soya agar)
 
[[SMM]] (supplemented liquid minimal medium) | [[SMMS]] (supplemented minimal medium, solid)
 
[[SNA]] (Soft nutrient agar)
 
[[SPY]] (Salt Peptone Yeast)
 
[[TSB]] (Tryptone Soy Broth)


[[YEME]] (Yeast Extract + Malt Extract)
[[YEME]] (Yeast Extract + Malt Extract)
[[YMPD]] (Yeast Meat Peptone Dextrose)
[[YMS]] (Yeast-Malt extract-Soluble starch medium)
[[YP]] (Yeast Peptone) | [[YPD]] (Yeast Peptone Dextrose)
[[2X YT]]
====<u>International ''Streptomyces'' Project Media (ISP's)</u>====
[[ISP2]]
[[ISP3]]
[[ISP4]]
[[ISP5]]
[[ISP6]]
[[ISP7]]
==Media Supplements==
[[Antibiotic stocks and working concentrations]]
[[Indicator compounds]]
[[Media supplements for activating cryptic biosynthetic gene clusters]]
[[Media supplements for induction gene expression from inducible promoters]]
[[Trace element solution]]

Latest revision as of 10:06, 2 March 2026


Media Recipes

Media Recipes

Please note that 15 or 20g/L (1.5-2%) agar is added to make solid medium

ABB13

A1

CM (Complete medium)

DNA (Difco Nutrient Agar) | DNB (Difco Nutrient Broth) | SNA (Soft Nutrient Agar)

Emerson medium

Gause's No. 1

GYM (Glucose + Yeast Extract + Malt Extract)

HT (Hickey-Tresner)

HV

IMA (Smash)

LB (Lysogeny Broth) | Lennox (Lennox agar) | Luria (Luria agar)

MC agar (Milk Casein Agar)

MM (Minimal medium)

MS (Mannitol Soya agar - a.k.a. Soya Flour Mannitol (SFM))

1/2 MS-S

1/2 MS+B5

MYM (Maltose + Yeast Extract + Malt Extract)

NMMP (minimal liquid medium)

OT (oligotrophic agar)

PDA (Potato Dextrose Agar)

R2 | R2YE | R5

SC (Starch Casein agar)

SFM (Soya Flour Mannitol - a.k.a. Mannitol Soya agar)

SMM (supplemented liquid minimal medium) | SMMS (supplemented minimal medium, solid)

SNA (Soft nutrient agar)

SPY (Salt Peptone Yeast)

TSB (Tryptone Soy Broth)

YEME (Yeast Extract + Malt Extract)

YMPD (Yeast Meat Peptone Dextrose)

YMS (Yeast-Malt extract-Soluble starch medium)

YP (Yeast Peptone) | YPD (Yeast Peptone Dextrose)

2X YT

International Streptomyces Project Media (ISP's)

ISP2

ISP3

ISP4

ISP5

ISP6

ISP7

Media Supplements

Antibiotic stocks and working concentrations

Indicator compounds

Media supplements for activating cryptic biosynthetic gene clusters

Media supplements for induction gene expression from inducible promoters

Trace element solution