Media recipes: Difference between revisions
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{{DISPLAYTITLE:Media Recipes}} | |||
[[File:Actinobase-icons-trans_Media-recipes.png|150px|Media Recipes|link=Media_recipes]] | [[File:Actinobase-icons-trans_Media-recipes.png|150px|Media Recipes|link=Media_recipes]] | ||
=Media Recipes= | |||
Please note that 15 or 20g/L (1.5-2%) agar is added to make solid medium | |||
[[ABB13]] | |||
[[A1]] | [[A1]] | ||
| Line 5: | Line 13: | ||
[[CM]] (Complete medium) | [[CM]] (Complete medium) | ||
[[DNA]] (Difco Nutrient Agar) | [[DNB]] (Difco Nutrient Broth) | [[SNA]] ( | [[DNA]] (Difco Nutrient Agar) | [[DNB]] (Difco Nutrient Broth) | [[SNA]] (Soft Nutrient Agar) | ||
[[Emerson medium]] | [[Emerson medium]] | ||
| Line 19: | Line 27: | ||
[[IMA]] (Smash) | [[IMA]] (Smash) | ||
[[ | [[LB]] (Lysogeny Broth) | [[Lennox]] (Lennox agar) | [[Luria]] (Luria agar) | ||
[[ | [[MC agar]] (Milk Casein Agar) | ||
[[ | [[MM]] (Minimal medium) | ||
[[ | [[SFM | MS]] (Mannitol Soya agar - a.k.a. Soya Flour Mannitol (SFM)) | ||
[[ | [[1/2 MS0 | 1/2 MS-S]] | ||
[[ | [[1/2 MS+B5]] | ||
[[MYM]] (Maltose + Yeast Extract + Malt Extract) | [[MYM]] (Maltose + Yeast Extract + Malt Extract) | ||
| Line 41: | Line 45: | ||
[[OT]] (oligotrophic agar) | [[OT]] (oligotrophic agar) | ||
[[ | [[PDA]] (Potato Dextrose Agar) | ||
[[R2]] | [[R2YE]] | [[R5]] | [[R2]] | [[R2YE]] | [[R5]] | ||
| Line 47: | Line 51: | ||
[[SC]] (Starch Casein agar) | [[SC]] (Starch Casein agar) | ||
[[SFM]] ( | [[SFM]] (Soya Flour Mannitol - a.k.a. Mannitol Soya agar) | ||
[[SMM]] (supplemented liquid minimal medium) | [[SMMS]] (supplemented minimal medium, solid) | [[SMM]] (supplemented liquid minimal medium) | [[SMMS]] (supplemented minimal medium, solid) | ||
[[SNA]] (Soft nutrient agar) | |||
[[SPY]] (Salt Peptone Yeast) | [[SPY]] (Salt Peptone Yeast) | ||
| Line 56: | Line 62: | ||
[[YEME]] (Yeast Extract + Malt Extract) | [[YEME]] (Yeast Extract + Malt Extract) | ||
[[YMPD]] (Yeast Meat Peptone Dextrose) | |||
[[YMS]] (Yeast-Malt extract-Soluble starch medium) | |||
[[YP]] (Yeast Peptone) | [[YPD]] (Yeast Peptone Dextrose) | [[YP]] (Yeast Peptone) | [[YPD]] (Yeast Peptone Dextrose) | ||
| Line 61: | Line 71: | ||
[[2X YT]] | [[2X YT]] | ||
[[ | ====<u>International ''Streptomyces'' Project Media (ISP's)</u>==== | ||
[[ISP2]] | |||
[[ISP3]] | |||
[[ISP4]] | |||
[[ISP5]] | |||
[[ISP6]] | |||
[[ISP7]] | |||
==Media Supplements== | |||
[[Antibiotic stocks and working concentrations]] | |||
[[Indicator compounds]] | |||
[[Media supplements for activating cryptic biosynthetic gene clusters]] | |||
[[Media supplements for induction gene expression from inducible promoters]] | |||
[[Trace element solution]] | |||
Latest revision as of 10:06, 2 March 2026
Media Recipes
Please note that 15 or 20g/L (1.5-2%) agar is added to make solid medium
CM (Complete medium)
DNA (Difco Nutrient Agar) | DNB (Difco Nutrient Broth) | SNA (Soft Nutrient Agar)
GYM (Glucose + Yeast Extract + Malt Extract)
HT (Hickey-Tresner)
IMA (Smash)
LB (Lysogeny Broth) | Lennox (Lennox agar) | Luria (Luria agar)
MC agar (Milk Casein Agar)
MM (Minimal medium)
MS (Mannitol Soya agar - a.k.a. Soya Flour Mannitol (SFM))
MYM (Maltose + Yeast Extract + Malt Extract)
NMMP (minimal liquid medium)
OT (oligotrophic agar)
PDA (Potato Dextrose Agar)
SC (Starch Casein agar)
SFM (Soya Flour Mannitol - a.k.a. Mannitol Soya agar)
SMM (supplemented liquid minimal medium) | SMMS (supplemented minimal medium, solid)
SNA (Soft nutrient agar)
SPY (Salt Peptone Yeast)
TSB (Tryptone Soy Broth)
YEME (Yeast Extract + Malt Extract)
YMPD (Yeast Meat Peptone Dextrose)
YMS (Yeast-Malt extract-Soluble starch medium)
YP (Yeast Peptone) | YPD (Yeast Peptone Dextrose)
International Streptomyces Project Media (ISP's)
Media Supplements
Antibiotic stocks and working concentrations
Media supplements for activating cryptic biosynthetic gene clusters
Media supplements for induction gene expression from inducible promoters
