Media recipes: Difference between revisions
Sam Prudence (talk | contribs) No edit summary |
|||
| (17 intermediate revisions by 7 users not shown) | |||
| Line 4: | Line 4: | ||
=Media Recipes= | =Media Recipes= | ||
Please note that 15 or 20g/L (1.5-2%) agar is added to make solid medium | |||
[[ABB13]] | |||
[[A1]] | [[A1]] | ||
| Line 23: | Line 27: | ||
[[IMA]] (Smash) | [[IMA]] (Smash) | ||
[[LB]] (Lysogeny Broth) | [[ | [[LB]] (Lysogeny Broth) | [[Lennox]] (Lennox agar) | [[Luria]] (Luria agar) | ||
[[MC agar]] (Milk Casein Agar) | [[MC agar]] (Milk Casein Agar) | ||
| Line 29: | Line 33: | ||
[[MM]] (Minimal medium) | [[MM]] (Minimal medium) | ||
[[SFM | MS]] (Mannitol Soya agar - a.k.a. Soya Flour Mannitol) | [[SFM | MS]] (Mannitol Soya agar - a.k.a. Soya Flour Mannitol (SFM)) | ||
[[1/2 MS0 | 1/2 MS-S]] | |||
[[1/2 MS+B5]] | |||
[[MYM]] (Maltose + Yeast Extract + Malt Extract) | [[MYM]] (Maltose + Yeast Extract + Malt Extract) | ||
| Line 47: | Line 55: | ||
[[SMM]] (supplemented liquid minimal medium) | [[SMMS]] (supplemented minimal medium, solid) | [[SMM]] (supplemented liquid minimal medium) | [[SMMS]] (supplemented minimal medium, solid) | ||
[[SNA]] (Soft nutrient agar) | |||
[[ | |||
[[SPY]] (Salt Peptone Yeast) | [[SPY]] (Salt Peptone Yeast) | ||
| Line 66: | Line 62: | ||
[[YEME]] (Yeast Extract + Malt Extract) | [[YEME]] (Yeast Extract + Malt Extract) | ||
[[YMPD]] (Yeast Meat Peptone Dextrose) | |||
[[YMS]] (Yeast-Malt extract-Soluble starch medium) | |||
[[YP]] (Yeast Peptone) | [[YPD]] (Yeast Peptone Dextrose) | [[YP]] (Yeast Peptone) | [[YPD]] (Yeast Peptone Dextrose) | ||
| Line 71: | Line 71: | ||
[[2X YT]] | [[2X YT]] | ||
<u> | ====<u>International ''Streptomyces'' Project Media (ISP's)</u>==== | ||
[[ISP2]] | [[ISP2]] | ||
| Line 94: | Line 94: | ||
[[Media supplements for induction gene expression from inducible promoters]] | [[Media supplements for induction gene expression from inducible promoters]] | ||
[[Trace element solution]] | |||
Latest revision as of 10:06, 2 March 2026
Media Recipes
Please note that 15 or 20g/L (1.5-2%) agar is added to make solid medium
CM (Complete medium)
DNA (Difco Nutrient Agar) | DNB (Difco Nutrient Broth) | SNA (Soft Nutrient Agar)
GYM (Glucose + Yeast Extract + Malt Extract)
HT (Hickey-Tresner)
IMA (Smash)
LB (Lysogeny Broth) | Lennox (Lennox agar) | Luria (Luria agar)
MC agar (Milk Casein Agar)
MM (Minimal medium)
MS (Mannitol Soya agar - a.k.a. Soya Flour Mannitol (SFM))
MYM (Maltose + Yeast Extract + Malt Extract)
NMMP (minimal liquid medium)
OT (oligotrophic agar)
PDA (Potato Dextrose Agar)
SC (Starch Casein agar)
SFM (Soya Flour Mannitol - a.k.a. Mannitol Soya agar)
SMM (supplemented liquid minimal medium) | SMMS (supplemented minimal medium, solid)
SNA (Soft nutrient agar)
SPY (Salt Peptone Yeast)
TSB (Tryptone Soy Broth)
YEME (Yeast Extract + Malt Extract)
YMPD (Yeast Meat Peptone Dextrose)
YMS (Yeast-Malt extract-Soluble starch medium)
YP (Yeast Peptone) | YPD (Yeast Peptone Dextrose)
International Streptomyces Project Media (ISP's)
Media Supplements
Antibiotic stocks and working concentrations
Media supplements for activating cryptic biosynthetic gene clusters
Media supplements for induction gene expression from inducible promoters
