User contributions for LisAlgora
From ActinoBase
23 April 2020
- 22:3722:37, 23 April 2020 diff hist +1,267 N Lambda DNA/HindIII Ladder Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate..." current
- 22:3622:36, 23 April 2020 diff hist 0 Southern Blotting →2. PCR Labelling current
- 22:3522:35, 23 April 2020 diff hist +1,267 N Lambda DNA/HindIII ladder Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate..." current
- 22:2322:23, 23 April 2020 diff hist +8,185 N Solutions for Southern Blotting Created page with "=Stock Solutions to Prepare in Advance= {| class="wikitable" ! style="font-family:Arial, Helvetica, sans-serif !important;; background-color:rgb(222, 234, 246); color:rgb(0,..." current
- 21:5521:55, 23 April 2020 diff hist +4,607 N Consumables for Southern Blotting Created page with "Roche provides a <b>DIG DNA Labelling and Detection Kit</b> that contains: blocking reagent, Anti-DIG-AP conjugate, Hexanucleotide Mix, DIG DNA Labelling Mix, Klenow Enzyme an..." current
- 21:1921:19, 23 April 2020 diff hist +6,785 Southern Blotting No edit summary
27 September 2019
- 14:3514:35, 27 September 2019 diff hist +2,947 N Southern Blotting Created page with "There are many different protocols for Southern blotting. This approach uses vacuum transfer pf DNA to a nylon membrane, DIG-labelling of the probe and detection by chromogeni..."
- 13:4513:45, 27 September 2019 diff hist +23 Nucleic Acid Methods No edit summary current
4 September 2019
- 14:1314:13, 4 September 2019 diff hist +4 Streptomyces clavuligerus No edit summary
- 14:1114:11, 4 September 2019 diff hist +2,279 Streptomyces clavuligerus No edit summary
- 13:2713:27, 4 September 2019 diff hist −358 R2YE No edit summary current
- 13:2513:25, 4 September 2019 diff hist +2 P buffer No edit summary current
- 13:2313:23, 4 September 2019 diff hist −24 P buffer No edit summary
- 13:2113:21, 4 September 2019 diff hist −2 R2 No edit summary
- 13:2113:21, 4 September 2019 diff hist +2 L buffer No edit summary current
- 13:2013:20, 4 September 2019 diff hist +341 N Trace element solution Created page with "'''Trace element solution (per liter):''' *40 mg ZnCl<sub>2</sub> *200 mg FeCl<sub>3</sub> . 6H<sub>2</sub>O *10 mg CuCl<sub>2</sub> . 2H<sub>2</sub>O *10 mg MnCl<sub>2</sub>..."
- 13:1813:18, 4 September 2019 diff hist −356 R2 No edit summary
- 13:1713:17, 4 September 2019 diff hist +516 N L buffer Created page with "'''L (lysis) buffer''' (Thompson ''et al.'' 1982) Mix the following sterile solutions: *100 ml Sucrose (10.3%) *10 ml TES buffer (5.73%, adjusted to pH7.2) *1 ml K<sub>2</su..."
- 12:5112:51, 4 September 2019 diff hist +513 N P buffer Created page with "'''P (protoplast) buffer''' (Okanishi ''et al.'', 1974; Hopwood and Wright, 1978) Make up the following basal solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *2...."
- 12:4012:40, 4 September 2019 diff hist +60 Cloning, Transformation and Expression of Gene Clusters No edit summary current
- 12:4012:40, 4 September 2019 diff hist −60 Protocols No edit summary
- 11:1411:14, 4 September 2019 diff hist +957 Conjugation using ET12567/pUZ8002 No edit summary
- 11:1311:13, 4 September 2019 diff hist +213 Protoplasts Formation, Regeneration and Transformation No edit summary
- 10:4910:49, 4 September 2019 diff hist +53 Conjugation using ET12567/pUZ8002 No edit summary
- 10:4610:46, 4 September 2019 diff hist +19 Conjugation using ET12567/pUZ8002 No edit summary
- 10:4510:45, 4 September 2019 diff hist +15 Conjugation using ET12567/pUZ8002 No edit summary
- 10:4310:43, 4 September 2019 diff hist +4 Staining of Streptomyces spp. Cell Wall and Nucleic Acids No edit summary current
- 10:3710:37, 4 September 2019 diff hist +1 Protoplasts Formation, Regeneration and Transformation No edit summary
- 10:3710:37, 4 September 2019 diff hist +1,379 Protoplasts Formation, Regeneration and Transformation No edit summary
30 August 2019
- 15:3115:31, 30 August 2019 diff hist −9 Protoplasts Formation, Regeneration and Transformation No edit summary
- 15:3015:30, 30 August 2019 diff hist +3 Protoplasts Formation, Regeneration and Transformation No edit summary
- 15:2815:28, 30 August 2019 diff hist +2,680 N Protoplasts Formation, Regeneration and Transformation Created page with "Protoplasts are used for fusion, transformation and transfection. =Protoplasts Preparation= Although this protocol was written for ''Streptomyces lividans'' and ''Str..."
- 14:2914:29, 30 August 2019 diff hist +60 Protocols No edit summary
- 14:1214:12, 30 August 2019 diff hist +102 PIJ6902 No edit summary
- 14:0714:07, 30 August 2019 diff hist +42 PIJ6902 No edit summary
- 14:0014:00, 30 August 2019 diff hist +4 PIJ6902 No edit summary
- 13:5913:59, 30 August 2019 diff hist 0 PIJ6902 No edit summary
- 13:5613:56, 30 August 2019 diff hist +88 PIJ6902 No edit summary
- 13:5313:53, 30 August 2019 diff hist 0 N File:PIJ6902.gif No edit summary current
- 13:4813:48, 30 August 2019 diff hist +368 N PIJ6902 Created page with "{{DISPLAYTITLE: pIJ6092}} ==Use== ==Features== *Apramycin resistance cassette *φBT1 integration site & integrase ==History== ==Map== ==Sequence links== ==References=..."
- 13:4313:43, 30 August 2019 diff hist +13 Plasmids No edit summary
26 July 2019
- 17:3317:33, 26 July 2019 diff hist +8 m SC No edit summary current
- 17:2617:26, 26 July 2019 diff hist +4 m Streptomyces No edit summary
- 17:2217:22, 26 July 2019 diff hist +5 ISP2 No edit summary current
- 17:1617:16, 26 July 2019 diff hist −3 R2 No edit summary
- 17:1617:16, 26 July 2019 diff hist +1,694 N R2YE Created page with "=R2YE= R2YE medium is a variation of R2 containing yeast extract. ==Preparation== Make up the following solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *10.12 g..."
- 17:0117:01, 26 July 2019 diff hist 0 R2 No edit summary
- 16:5916:59, 26 July 2019 diff hist +52 ISP3 No edit summary current
- 16:5816:58, 26 July 2019 diff hist +4 ISP2 No edit summary
- 16:5716:57, 26 July 2019 diff hist +12 ISP2 No edit summary
- 16:5516:55, 26 July 2019 diff hist −1 SFM No edit summary
- 16:5216:52, 26 July 2019 diff hist 0 Media recipes No edit summary
- 16:0516:05, 26 July 2019 diff hist −1 R2 No edit summary
- 16:0416:04, 26 July 2019 diff hist −5 R2 No edit summary
- 16:0316:03, 26 July 2019 diff hist −4 R2 No edit summary
- 16:0216:02, 26 July 2019 diff hist +6 R2 No edit summary
- 16:0116:01, 26 July 2019 diff hist +1,795 N R2 Created page with "=R2= ==Preparation== #Make up the following solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *10.12 g MgCl<sub>2</sub>.6H<sub>2</sub>O *10 g Glucose *0.1 g Difc..."
- 15:1115:11, 26 July 2019 diff hist +8 MC agar No edit summary
- 15:1015:10, 26 July 2019 diff hist +8 MC agar No edit summary
- 15:0615:06, 26 July 2019 diff hist +21 SNA No edit summary
- 14:5514:55, 26 July 2019 diff hist −4 SNA No edit summary
- 14:5314:53, 26 July 2019 diff hist +134 SNA No edit summary
- 14:5014:50, 26 July 2019 diff hist +40 SFM No edit summary
- 14:4614:46, 26 July 2019 diff hist +490 N SNA Created page with "=SNA= Soft Nutrient Agar (SNA) ==Preparation== Per Litre: *4g Difco Nutrient Broth Powder *5g Agar *1L dH<sub>2</sub>O '''Instructions''' #Add 0.8g of DNB powder into eac..."
- 14:3714:37, 26 July 2019 diff hist +4 DNA No edit summary
- 14:3214:32, 26 July 2019 diff hist +19 2X YT No edit summary
- 14:2914:29, 26 July 2019 diff hist +184 2X YT No edit summary
- 14:2814:28, 26 July 2019 diff hist +8 SFM No edit summary
- 14:2414:24, 26 July 2019 diff hist +276 N 2X YT Created page with "=2x YT= ==Preparation== Per litre: *16 g Tryptone *10 g Yeast Extract *5 g NaCl *1 L dH<sub>2</sub>O '''Instructions''' #Dissolve constituents in 1L dH<sub>2</sub>O #Ste..."
