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- 15:35, 1 November 2023 RTEvans talk contribs created page File:PanelB.png (Genome mapping of S. coelicolor samples following rRNA depletion using NEBNext rRNA Depletion Kit (Bacteria))
- 15:35, 1 November 2023 RTEvans talk contribs uploaded File:PanelB.png (Genome mapping of S. coelicolor samples following rRNA depletion using NEBNext rRNA Depletion Kit (Bacteria))
- 15:33, 1 November 2023 RTEvans talk contribs created page File:PanelA.png (Genome mapping of S. coelicolor samples following rRNA depletion with Qiagen FastSelect 5S/16S/23S kit)
- 15:33, 1 November 2023 RTEvans talk contribs uploaded File:PanelA.png (Genome mapping of S. coelicolor samples following rRNA depletion with Qiagen FastSelect 5S/16S/23S kit)
- 15:26, 1 November 2023 RTEvans talk contribs created page File:RRNA depletion table 1.png (Table 1 - list of abundant non mRNA RNAs in S. coelicolor A3(2))
- 15:26, 1 November 2023 RTEvans talk contribs uploaded File:RRNA depletion table 1.png (Table 1 - list of abundant non mRNA RNAs in S. coelicolor A3(2))
- 15:25, 1 November 2023 RTEvans talk contribs created page RRNA depletion with Streptomyces coelicolor A3(2) specific probes (Created page with "==Organisms== This protocol has been tested with <em>Streptomyces coelicolor</em> A3(2) MT1110 ==rRNA depletion with <em>Streptomyces coelicolor</em> A3(2) specific probes== rRNA depletion using commercial kits targeting generic bacterial sequences is not 100% efficient in the High GC organism S. coelicolor. Depletion using the NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads (https://www.neb.com/en-gb/products/e7870-nebnext-rna-depleti...")
- 11:26, 1 November 2023 User account RTEvans talk contribs was created by MFeeney talk contribs (new user account)
- 07:36, 11 October 2023 User account Gilles talk contribs was created by Matt Hutchings talk contribs
- 17:01, 28 September 2023 User account Mgonza local talk contribs was created automatically
- 16:16, 12 September 2023 Matt Hutchings talk contribs undeleted page CRISPR (5 revisions)
- 15:59, 12 September 2023 Matt Hutchings talk contribs created page MtrA methods are here (Created page with "Som N, Devine R, Chandra G, Martins C, Wilkinson B and Hutchings MI (2023). MtrA regulates the expression of developmental genes, including BldM and WhiI, in Streptomyces vene...")
- 15:57, 12 September 2023 Matt Hutchings talk contribs created page Methods (Created page with "This allows you to publish supplementary methods to support, and cite, in your publications. Email matt.hutchings@jic.ac.uk Som N, Devine R, Chandra G, Martins C, Wilkinson...")
- 07:52, 21 August 2023 Matt Hutchings talk contribs created page VProtocols (Created page with "Below are links to video protocols created by our members: Streptomyces spore preparation by Lorena Fernández-Martínez <Fernandl@edgehill.ac.uk>")
- 09:18, 30 June 2023 User account Tomgreg talk contribs was created by Matt Hutchings talk contribs
- 07:45, 30 June 2023 User account Thomgregory talk contribs was created by Matt Hutchings talk contribs
- 08:18, 29 June 2023 User account Thomgreg talk contribs was created by Matt Hutchings talk contribs and password was sent by email (New editor)
- 13:41, 13 September 2022 User account LabInsubria talk contribs was created by MFeeney talk contribs
- 10:01, 28 July 2022 User account Nmvior talk contribs was created by MFeeney talk contribs
- 15:11, 21 July 2022 User account Fernandl talk contribs was created by MFeeney talk contribs (password reset not working)
- 14:32, 20 July 2022 User account Lorena talk contribs was created by MFeeney talk contribs and password was sent by email
- 12:35, 28 March 2022 Jake Newitt talk contribs created page Wheat Take-all bioassays (Created page with "=Wheat Take-all bioassays= This assay is used to determine whether ''Streptomyces'' strains are able to produce antifungal compounds that inhibit the wheat root pathogen ''Ga...")
- 09:05, 28 March 2022 Jake Newitt talk contribs created page Imaging GFP streptomyces on arabidopsis roots (Created page with "=Imaging GFP streptomyces on ''Arabidopsis'' roots= ''Streptomyces'' strains that contain pIJ8660/PermE* and are able to produce the eGFP protein encoded by it can be visuali...")
- 21:50, 20 March 2022 MFeeney talk contribs created page SV1 transduction (Created page with "=<strong>Phage transduction using SV1</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces venezuelae</em> with ΦSV1 ===Ph...")
- 16:38, 12 November 2021 User account Nelly talk contribs was created by Matt Hutchings talk contribs (New editor)
- 18:01, 3 November 2021 Sam Prudence talk contribs changed group membership for Agustina Undabarrena from (none) to administrator, interface administrator and bureaucrat
- 18:00, 3 November 2021 User account Agustina Undabarrena talk contribs was created by Sam Prudence talk contribs
- 14:18, 2 November 2021 Damien Gayrard talk contribs created page 1/2 MS+B5 (Created page with "==Preparation== Per Litre: *2.205 MS Salts and Gamborg B5 Vitamins (Duchefa M0231) *1 L ddH2O *5M KOH *Bacto Agar '''Instructions''' #Dissolve 2.2g MS Salts + Gamborg B5 Vit...")
- 11:09, 2 November 2021 User account Damien Gayrard talk contribs was created by Jake Newitt talk contribs
- 12:31, 29 October 2021 Louisa perry jic talk contribs created page 1/2 MS0 (Created page with "==Preparation== Per Litre: *2.205 g g Murashige & Skoog Medium Without Vitamins *1 L tap water *NaOH '''Instructions''' #Dissolve 2.205g Murashige & Skoog in 1L tap water #...")
- 12:24, 29 October 2021 Louisa perry jic talk contribs created page Wheat root exudate harvesting (Created page with "{{DISPLAYTITLE: Wheat root exudate harvesting}} <h1>Wheat root exudates and the recruitment of root microbes</h1> ... <h1>You will need:</h1> Preparation of growth tubes * 5...")
- 14:25, 26 October 2021 Jake Newitt talk contribs created page Wheat seed sterilisation (Created page with "{{DISPLAYTITLE: Wheat seed sterilisation}} =Wheat seed sterilisation protocol= Wheat seeds are typically sterilised before growing in sterile conditions, such is the case for...")
- 14:06, 26 October 2021 Louisa perry jic talk contribs created page MS+B5 (Created page with "==Preparation== Per Litre: *2.25 g g Murashige & Skoog Medium Without Vitamins *0.056 g Gamborg B5 Vitamin Mixture *12 g Agar *1 L tap water '''Instructions''' #Dissolve 2.25...")
- 13:24, 26 October 2021 Louisa perry jic talk contribs created page Growing Marchantia from gemma spores (Created page with "{{DISPLAYTITLE: Growing Marchantia from gemma spores}} ==Marchantia and their lifecycle== Marchantia are a genus of plant in the Bryophyte taxonomic division along with mosse...")
- 12:46, 26 October 2021 User account Louisa perry jic talk contribs was created by Jake Newitt talk contribs (new account)
- 12:44, 26 October 2021 User account Louisa perry talk contribs was created by Jake Newitt talk contribs and password was sent by email (new account)
- 11:45, 26 October 2021 User account Louisa Perry talk contribs was created by Jake Newitt talk contribs and password was sent by email (Creating account for colleague)
- 08:03, 21 October 2021 Matt Hutchings talk contribs created page Plant-Microbe interactions (Created page with "In recent years it has become clear that actinomycetes like Streptomyces species are not simply free-living soil bacteria. They also interact with plant roots and can colonise...")
- 07:53, 21 October 2021 Matt Hutchings talk contribs created page Plants (Created page with "150px|Protocols|link=Protocols =Protocols organised by category= ==Bacteriophage== *Streptomyces Bacteriophage plaque a...")
- 16:23, 20 October 2021 Sam Prudence talk contribs changed group membership for Kate Duncan from (none) to administrator, interface administrator and bureaucrat
- 16:21, 20 October 2021 User account Kate Duncan talk contribs was created by Sam Prudence talk contribs
- 16:02, 20 October 2021 Sam Prudence talk contribs changed group membership for Jake Newitt from (none) to administrator, interface administrator and bureaucrat
- 16:01, 20 October 2021 User account Jake Newitt talk contribs was created by Sam Prudence talk contribs
- 14:59, 20 October 2021 User account Sergey Group talk contribs was created by Sam Prudence talk contribs
- 14:57, 20 October 2021 Sam Prudence talk contribs changed group membership for J Newitt from (none) to administrator, interface administrator and bureaucrat
- 14:53, 20 October 2021 Sam Prudence talk contribs changed group membership for Matt Hutchings from (none) to administrator, interface administrator and bureaucrat
- 13:14, 28 July 2021 User account Matt Hutchings talk contribs was created by Sam Prudence talk contribs
- 16:26, 18 May 2021 H Otani talk contribs created page PCRISPR-dCas9 (Created page with "==References== #Tong Y, Charusanti P, Zhang L, Weber T, Lee SY. CRISPR-Cas9 Based Engineering of Actinomycetal Genomes. ''ACS Synth Biol'' 2015 Sep 18;4(9):1020-9. doi: 10.102...")
- 16:25, 18 May 2021 H Otani talk contribs created page PCRISPR-Cas9 (Created page with "==References== #Tong Y, Charusanti P, Zhang L, Weber T, Lee SY. CRISPR-Cas9 Based Engineering of Actinomycetal Genomes. ''ACS Synth Biol'' 2015 Sep 18;4(9):1020-9. doi: 10.102...")
- 16:23, 18 May 2021 H Otani talk contribs created page PTYM18 (Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector...")
- 16:22, 18 May 2021 H Otani talk contribs created page PTYM19 (Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector...")
- 16:22, 18 May 2021 H Otani talk contribs created page PTOYAMAcos (Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector...")
- 10:01, 26 April 2021 User account S Jarmusch talk contribs was created by Sam Prudence talk contribs
- 11:48, 5 January 2021 User account Parva Zarei talk contribs was created by Sam Prudence talk contribs
- 08:59, 18 August 2020 Alicia talk contribs created page Rhodococcus erythropolis (Created page with "==Organism Page== === ''Rhodococcus erythropolis'' === ===Background=== ===Growth & Maintenance=== GYM =====Sporulation===== Nonsporulating ===Strains=== <em>Rhodo...")
- 12:27, 6 June 2020 Sam Prudence talk contribs created page File:Graphical overview LFM.png
- 12:27, 6 June 2020 Sam Prudence talk contribs uploaded File:Graphical overview LFM.png
- 12:23, 6 June 2020 Sam Prudence talk contribs created page File:LFM plasmid maps.png
- 12:23, 6 June 2020 Sam Prudence talk contribs uploaded File:LFM plasmid maps.png
- 12:07, 6 June 2020 Sam Prudence talk contribs created page Meganuclease I-SceI based system for gene deletions (Created page with "=Gene deletion in actinomycetes using I-SceI meganuclease= This protocol allows the reader to make clean deletions in any actinomycete species using an efficient method to gen...")
- 11:54, 6 June 2020 Sam Prudence talk contribs created page Microbispora (Created page with "=<i>Microbispora</i>= Actinobacteria / Actinobacteria / Streptosporangiales / Streptosporangiaceae / <i>Microbispora</i> <i>Microbispora</i> is a genus of aerobic, Gram-posit...")
- 11:53, 6 June 2020 Sam Prudence talk contribs created page File:Microbispora.png
- 11:53, 6 June 2020 Sam Prudence talk contribs uploaded File:Microbispora.png
- 11:04, 6 May 2020 MFeeney talk contribs created page File:SsDNA recombineering oligos.png (oligonucleotide design for ssDNA recombineering)
- 11:04, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA recombineering oligos.png (oligonucleotide design for ssDNA recombineering)
- 10:16, 6 May 2020 MFeeney talk contribs created page File:SsDNA recombineering protocol.png (figure illustrating two key steps in the ssDNA recombineering protocol)
- 10:16, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA recombineering protocol.png (figure illustrating two key steps in the ssDNA recombineering protocol)
- 10:13, 6 May 2020 MFeeney talk contribs created page File:SsDNA diagnostic pcr.png (description of restriction polymorphism and mismatch PCR approaches to identify the mutant allele generated by ssDNA recombineering)
- 10:13, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA diagnostic pcr.png (description of restriction polymorphism and mismatch PCR approaches to identify the mutant allele generated by ssDNA recombineering)
- 11:07, 5 May 2020 MFeeney talk contribs created page Isolation of phages from environmental samples (Created page with "=<strong>Isolation of phages from environmental samples</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces lividans</em> s...")
- 10:44, 5 May 2020 MFeeney talk contribs created page Isolation of phages from single plaques (Created page with "=<strong>Isolation of phages from single plaques</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</em> with...")
- 10:09, 5 May 2020 MFeeney talk contribs created page High titre preparation of phage (Created page with "=<strong>High titre preparation of ''Streptomyces'' phage</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</e...")
- 09:47, 5 May 2020 MFeeney talk contribs created page File:PhiC31 serial dilution.jpg (Plaque assay: serial dilution of phiC31 on pglW- S. coelicolor)
- 09:47, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 serial dilution.jpg (Plaque assay: serial dilution of phiC31 on pglW- S. coelicolor)
- 09:02, 5 May 2020 MFeeney talk contribs created page Streptomyces Bacteriophage plaque assay (Created page with "=<strong>Streptomyces bacteriophage plaque assay</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</em> with...")
- 08:34, 5 May 2020 MFeeney talk contribs created page File:PhiC31 plaque assay.jpg (PhiC31 plaque assay on a pglW- strain of S. coelicolor)
- 08:34, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 plaque assay.jpg (PhiC31 plaque assay on a pglW- strain of S. coelicolor)
- 08:31, 5 May 2020 MFeeney talk contribs deleted page File:PhiC31 serial dilution.jpg
- 08:31, 5 May 2020 MFeeney talk contribs created page File:PhiC31 serial dilution.jpg (PhiC31 plaque assay)
- 08:31, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 serial dilution.jpg (PhiC31 plaque assay)
- 22:37, 23 April 2020 LisAlgora talk contribs created page Lambda DNA/HindIII Ladder (Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate...")
- 22:35, 23 April 2020 LisAlgora talk contribs created page Lambda DNA/HindIII ladder (Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate...")
- 22:23, 23 April 2020 LisAlgora talk contribs created page Solutions for Southern Blotting (Created page with "=Stock Solutions to Prepare in Advance= {| class="wikitable" ! style="font-family:Arial, Helvetica, sans-serif !important;; background-color:rgb(222, 234, 246); color:rgb(0,...")
- 21:55, 23 April 2020 LisAlgora talk contribs created page Consumables for Southern Blotting (Created page with "Roche provides a <b>DIG DNA Labelling and Detection Kit</b> that contains: blocking reagent, Anti-DIG-AP conjugate, Hexanucleotide Mix, DIG DNA Labelling Mix, Klenow Enzyme an...")
- 09:03, 23 April 2020 MFeeney talk contribs moved page Bacteriophage to Streptomyces Bacteriophages without leaving a redirect
- 08:45, 23 April 2020 MFeeney talk contribs created page Bacteriophage (Created page with "=Streptomyces bacteriophages= Prof Paul A Hoskisson, University of Strathclyde. ==Introduction== Bacteriophages (or phages) are amongst the most abundant biological entities...")
- 12:51, 17 April 2020 Sam Prudence talk contribs changed group membership for MHutchings4 from (none) to administrator, interface administrator and bureaucrat
- 12:49, 17 April 2020 User account MHutchings4 talk contribs was created by Sam Prudence talk contribs
- 15:34, 16 April 2020 DaveMark talk contribs created page The ActinoBase e-Seminar (Created page with "=The ActinoBase e-Seminar Series= The ActinoBase e-Seminar Series is an opportunity for both early-career and established researchers in the Actinobacteria community to share...")
- 00:07, 25 March 2020 H Otani talk contribs created page RNA Sequencing (Created page with "#Extract and purify RNA samples according to RNA extraction from Actinobacteria. Add [https://www.thermofisher.com/order/catalog/product/4456740#/4456740 ERCC RNA Spike-In...")
- 23:43, 24 March 2020 H Otani talk contribs created page Natural Products (Created page with "Cloning, Transformation and Expression of Gene Clusters Metabolite extractions Production of microbial volatile organic compounds")
- 21:55, 24 March 2020 H Otani talk contribs created page Protoplast Transformation (Created page with "#Follow the instruction in Protoplasts Formation, Regeneration and Transformation #Add 3.85 ml P buffer to 1 g PEG sterilised. #Add 200 µl PEG-P buffer mixture and a DNA...")
- 17:27, 10 March 2020 Sam Prudence talk contribs created page File:Logo4.png
- 17:27, 10 March 2020 Sam Prudence talk contribs uploaded File:Logo4.png
- 17:17, 10 March 2020 Sam Prudence talk contribs created page File:ActinobaseLogo1.gif
- 17:17, 10 March 2020 Sam Prudence talk contribs uploaded File:ActinobaseLogo1.gif
- 15:09, 28 February 2020 MFeeney talk contribs created page Indicator compounds (Created page with "=Indicator Compounds= ==5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)== '''Uses''' *Detection of β-galactosidase activity on solid media *Bacterial two-hybrid a...")
- 14:33, 28 February 2020 DaveMark talk contribs created page File:PIJ12738 Map.png (Map of PIJ2738)
- 14:33, 28 February 2020 DaveMark talk contribs uploaded File:PIJ12738 Map.png (Map of PIJ2738)
- 14:06, 28 February 2020 User account Admb talk contribs was created by DaveMark talk contribs