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From ActinoBase
Combined display of all available logs of ActinoBase. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 12:46, 26 October 2021 User account Louisa perry jic talk contribs was created by Jake Newitt talk contribs (new account)
- 12:44, 26 October 2021 User account Louisa perry talk contribs was created by Jake Newitt talk contribs and password was sent by email (new account)
- 11:45, 26 October 2021 User account Louisa Perry talk contribs was created by Jake Newitt talk contribs and password was sent by email (Creating account for colleague)
- 08:03, 21 October 2021 Matt Hutchings talk contribs created page Plant-Microbe interactions (Created page with "In recent years it has become clear that actinomycetes like Streptomyces species are not simply free-living soil bacteria. They also interact with plant roots and can colonise...")
- 07:53, 21 October 2021 Matt Hutchings talk contribs created page Plants (Created page with "150px|Protocols|link=Protocols =Protocols organised by category= ==Bacteriophage== *Streptomyces Bacteriophage plaque a...")
- 16:23, 20 October 2021 Sam Prudence talk contribs changed group membership for Kate Duncan from (none) to administrator, interface administrator and bureaucrat
- 16:21, 20 October 2021 User account Kate Duncan talk contribs was created by Sam Prudence talk contribs
- 16:02, 20 October 2021 Sam Prudence talk contribs changed group membership for Jake Newitt from (none) to administrator, interface administrator and bureaucrat
- 16:01, 20 October 2021 User account Jake Newitt talk contribs was created by Sam Prudence talk contribs
- 14:59, 20 October 2021 User account Sergey Group talk contribs was created by Sam Prudence talk contribs
- 14:57, 20 October 2021 Sam Prudence talk contribs changed group membership for J Newitt from (none) to administrator, interface administrator and bureaucrat
- 14:53, 20 October 2021 Sam Prudence talk contribs changed group membership for Matt Hutchings from (none) to administrator, interface administrator and bureaucrat
- 13:14, 28 July 2021 User account Matt Hutchings talk contribs was created by Sam Prudence talk contribs
- 16:26, 18 May 2021 H Otani talk contribs created page PCRISPR-dCas9 (Created page with "==References== #Tong Y, Charusanti P, Zhang L, Weber T, Lee SY. CRISPR-Cas9 Based Engineering of Actinomycetal Genomes. ''ACS Synth Biol'' 2015 Sep 18;4(9):1020-9. doi: 10.102...")
- 16:25, 18 May 2021 H Otani talk contribs created page PCRISPR-Cas9 (Created page with "==References== #Tong Y, Charusanti P, Zhang L, Weber T, Lee SY. CRISPR-Cas9 Based Engineering of Actinomycetal Genomes. ''ACS Synth Biol'' 2015 Sep 18;4(9):1020-9. doi: 10.102...")
- 16:23, 18 May 2021 H Otani talk contribs created page PTYM18 (Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector...")
- 16:22, 18 May 2021 H Otani talk contribs created page PTYM19 (Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector...")
- 16:22, 18 May 2021 H Otani talk contribs created page PTOYAMAcos (Created page with "==References== #Onaka, H., Taniguchi, S., Ikeda, H., Igarashi, Y., and Furumai, T. (2003). pTOYAMAcos, pTYM18, and pTYM19, actinomycete-''Escherichia coli'' integrating vector...")
- 10:01, 26 April 2021 User account S Jarmusch talk contribs was created by Sam Prudence talk contribs
- 11:48, 5 January 2021 User account Parva Zarei talk contribs was created by Sam Prudence talk contribs
- 08:59, 18 August 2020 Alicia talk contribs created page Rhodococcus erythropolis (Created page with "==Organism Page== === ''Rhodococcus erythropolis'' === ===Background=== ===Growth & Maintenance=== GYM =====Sporulation===== Nonsporulating ===Strains=== <em>Rhodo...")
- 12:27, 6 June 2020 Sam Prudence talk contribs created page File:Graphical overview LFM.png
- 12:27, 6 June 2020 Sam Prudence talk contribs uploaded File:Graphical overview LFM.png
- 12:23, 6 June 2020 Sam Prudence talk contribs created page File:LFM plasmid maps.png
- 12:23, 6 June 2020 Sam Prudence talk contribs uploaded File:LFM plasmid maps.png
- 12:07, 6 June 2020 Sam Prudence talk contribs created page Meganuclease I-SceI based system for gene deletions (Created page with "=Gene deletion in actinomycetes using I-SceI meganuclease= This protocol allows the reader to make clean deletions in any actinomycete species using an efficient method to gen...")
- 11:54, 6 June 2020 Sam Prudence talk contribs created page Microbispora (Created page with "=<i>Microbispora</i>= Actinobacteria / Actinobacteria / Streptosporangiales / Streptosporangiaceae / <i>Microbispora</i> <i>Microbispora</i> is a genus of aerobic, Gram-posit...")
- 11:53, 6 June 2020 Sam Prudence talk contribs created page File:Microbispora.png
- 11:53, 6 June 2020 Sam Prudence talk contribs uploaded File:Microbispora.png
- 11:04, 6 May 2020 MFeeney talk contribs created page File:SsDNA recombineering oligos.png (oligonucleotide design for ssDNA recombineering)
- 11:04, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA recombineering oligos.png (oligonucleotide design for ssDNA recombineering)
- 10:16, 6 May 2020 MFeeney talk contribs created page File:SsDNA recombineering protocol.png (figure illustrating two key steps in the ssDNA recombineering protocol)
- 10:16, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA recombineering protocol.png (figure illustrating two key steps in the ssDNA recombineering protocol)
- 10:13, 6 May 2020 MFeeney talk contribs created page File:SsDNA diagnostic pcr.png (description of restriction polymorphism and mismatch PCR approaches to identify the mutant allele generated by ssDNA recombineering)
- 10:13, 6 May 2020 MFeeney talk contribs uploaded File:SsDNA diagnostic pcr.png (description of restriction polymorphism and mismatch PCR approaches to identify the mutant allele generated by ssDNA recombineering)
- 11:07, 5 May 2020 MFeeney talk contribs created page Isolation of phages from environmental samples (Created page with "=<strong>Isolation of phages from environmental samples</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces lividans</em> s...")
- 10:44, 5 May 2020 MFeeney talk contribs created page Isolation of phages from single plaques (Created page with "=<strong>Isolation of phages from single plaques</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</em> with...")
- 10:09, 5 May 2020 MFeeney talk contribs created page High titre preparation of phage (Created page with "=<strong>High titre preparation of ''Streptomyces'' phage</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</e...")
- 09:47, 5 May 2020 MFeeney talk contribs created page File:PhiC31 serial dilution.jpg (Plaque assay: serial dilution of phiC31 on pglW- S. coelicolor)
- 09:47, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 serial dilution.jpg (Plaque assay: serial dilution of phiC31 on pglW- S. coelicolor)
- 09:02, 5 May 2020 MFeeney talk contribs created page Streptomyces Bacteriophage plaque assay (Created page with "=<strong>Streptomyces bacteriophage plaque assay</strong>= This protocol has been confirmed to work for the following organisms: *<em>Streptomyces coelicolor</em> with...")
- 08:34, 5 May 2020 MFeeney talk contribs created page File:PhiC31 plaque assay.jpg (PhiC31 plaque assay on a pglW- strain of S. coelicolor)
- 08:34, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 plaque assay.jpg (PhiC31 plaque assay on a pglW- strain of S. coelicolor)
- 08:31, 5 May 2020 MFeeney talk contribs deleted page File:PhiC31 serial dilution.jpg
- 08:31, 5 May 2020 MFeeney talk contribs created page File:PhiC31 serial dilution.jpg (PhiC31 plaque assay)
- 08:31, 5 May 2020 MFeeney talk contribs uploaded File:PhiC31 serial dilution.jpg (PhiC31 plaque assay)
- 22:37, 23 April 2020 LisAlgora talk contribs created page Lambda DNA/HindIII Ladder (Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate...")
- 22:35, 23 April 2020 LisAlgora talk contribs created page Lambda DNA/HindIII ladder (Created page with "=Lambda HindIII Marker Preparation= *Lambda (λ) DNA (500 µg/ml): 100µl *10x Reaction Buffer: 45 µl *HindIII or PstI(10U/µl): 5µl *ddWater: 295µl Preparation: #Incubate...")
- 22:23, 23 April 2020 LisAlgora talk contribs created page Solutions for Southern Blotting (Created page with "=Stock Solutions to Prepare in Advance= {| class="wikitable" ! style="font-family:Arial, Helvetica, sans-serif !important;; background-color:rgb(222, 234, 246); color:rgb(0,...")
- 21:55, 23 April 2020 LisAlgora talk contribs created page Consumables for Southern Blotting (Created page with "Roche provides a <b>DIG DNA Labelling and Detection Kit</b> that contains: blocking reagent, Anti-DIG-AP conjugate, Hexanucleotide Mix, DIG DNA Labelling Mix, Klenow Enzyme an...")
- 09:03, 23 April 2020 MFeeney talk contribs moved page Bacteriophage to Streptomyces Bacteriophages without leaving a redirect
- 08:45, 23 April 2020 MFeeney talk contribs created page Bacteriophage (Created page with "=Streptomyces bacteriophages= Prof Paul A Hoskisson, University of Strathclyde. ==Introduction== Bacteriophages (or phages) are amongst the most abundant biological entities...")
- 12:51, 17 April 2020 Sam Prudence talk contribs changed group membership for MHutchings4 from (none) to administrator, interface administrator and bureaucrat
- 12:49, 17 April 2020 User account MHutchings4 talk contribs was created by Sam Prudence talk contribs
- 15:34, 16 April 2020 DaveMark talk contribs created page The ActinoBase e-Seminar (Created page with "=The ActinoBase e-Seminar Series= The ActinoBase e-Seminar Series is an opportunity for both early-career and established researchers in the Actinobacteria community to share...")
- 00:07, 25 March 2020 H Otani talk contribs created page RNA Sequencing (Created page with "#Extract and purify RNA samples according to RNA extraction from Actinobacteria. Add [https://www.thermofisher.com/order/catalog/product/4456740#/4456740 ERCC RNA Spike-In...")
- 23:43, 24 March 2020 H Otani talk contribs created page Natural Products (Created page with "Cloning, Transformation and Expression of Gene Clusters Metabolite extractions Production of microbial volatile organic compounds")
- 21:55, 24 March 2020 H Otani talk contribs created page Protoplast Transformation (Created page with "#Follow the instruction in Protoplasts Formation, Regeneration and Transformation #Add 3.85 ml P buffer to 1 g PEG sterilised. #Add 200 µl PEG-P buffer mixture and a DNA...")
- 17:27, 10 March 2020 Sam Prudence talk contribs created page File:Logo4.png
- 17:27, 10 March 2020 Sam Prudence talk contribs uploaded File:Logo4.png
- 17:17, 10 March 2020 Sam Prudence talk contribs created page File:ActinobaseLogo1.gif
- 17:17, 10 March 2020 Sam Prudence talk contribs uploaded File:ActinobaseLogo1.gif
- 15:09, 28 February 2020 MFeeney talk contribs created page Indicator compounds (Created page with "=Indicator Compounds= ==5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal)== '''Uses''' *Detection of β-galactosidase activity on solid media *Bacterial two-hybrid a...")
- 14:33, 28 February 2020 DaveMark talk contribs created page File:PIJ12738 Map.png (Map of PIJ2738)
- 14:33, 28 February 2020 DaveMark talk contribs uploaded File:PIJ12738 Map.png (Map of PIJ2738)
- 14:06, 28 February 2020 User account Admb talk contribs was created by DaveMark talk contribs
- 15:31, 20 February 2020 User account GenomeBrowser talk contribs was created by Sam Prudence talk contribs
- 15:26, 20 February 2020 Sam Prudence talk contribs moved page Salinospora to Salinispora
- 15:22, 20 February 2020 Sam Prudence talk contribs created page Genome browser (Created page with "<h2><strong>ActinoBase Genome Browser</strong></h2> Click the link below for the ActinoBase MORF Genome Browser, with antiSMASH integration. This tool has been kindly provide...")
- 19:50, 16 February 2020 JoshKlein talk contribs created page File:BioArtElvis.PNG (BioArt photo from https://doi.org/10.1371/journal.pbio.1000510.)
- 19:50, 16 February 2020 JoshKlein talk contribs uploaded File:BioArtElvis.PNG (BioArt photo from https://doi.org/10.1371/journal.pbio.1000510.)
- 19:49, 16 February 2020 JoshKlein talk contribs created page File:BioArt.png (A picture of various BioArt plates.)
- 19:49, 16 February 2020 JoshKlein talk contribs uploaded File:BioArt.png (A picture of various BioArt plates.)
- 15:27, 31 January 2020 ElmiraM talk contribs created page Streptacidiphilus (Created page with "=<i>Streptacidiphilus</i>= Streptacidiphilus (etymology: twisted, acid-loving) are one of the three genera belonging to the <i>Streptomycetaceae<i> family, along with <i>Strep...")
- 15:23, 31 January 2020 MFeeney talk contribs created page Lambda-red mediated recombination using ssDNA (Created page with "=Lambda-red mediated recombination using ssDNA= Lambda-red mediated recombination using ssDNA can be used to introduce precise mutations into the genome of <em>Streptomyces</e...")
- 14:47, 31 January 2020 Jordanmurray talk contribs created page Cultivating Actinobacteria for Microscopic Analysis (Created page with "In order to analyse actinobacteria using light microscopy, methods must take into account the multicellular nature of the test organism. Unlike unicellular organisms, actinoba...")
- 16:31, 29 January 2020 R Devine talk contribs created page ChIP Sequencing (Created page with "==General overview== Chromatin Immunoprecipitation and sequencing can be used to characterise protein-DNA interactions. It works by cross-linking proteins to DNA, shearing t...")
- 16:56, 14 November 2019 Sam Prudence talk contribs deleted page Trace elements (content was: "=Trace Elements= Recipe not added", and the only contributor was "Test" (talk))
- 17:29, 7 November 2019 Sam Prudence talk contribs deleted page CRISPR (content before blanking was: "Hutchings Lab protocol for generating CRISPR/Cas Knockouts using pCRISPomyces-2. Written by Rebecca Devine, Matt Hutchings’ Laboratory, University of East Anglia, UK <strong>Overview (see following pages for more deta...")
- 14:19, 25 October 2019 DaveMark talk contribs created page Isolating Actinobacteria from soil environments (Created page with "Isolating Actinobacteria from soil environments =Equipment & Materials= *Sterile containers to transport the soil *A heat block *Plates of Isolation media *A vortexer *¼ Str...")
- 14:08, 25 October 2019 LeightonP talk contribs created page Using pandoc (add page describing use of pandoc to convert Word documents to MediaWiki format)
- 14:01, 25 October 2019 LeightonP talk contribs created page File:Example word file.png (Screenshot of example Word file, before pandoc conversion)
- 14:01, 25 October 2019 LeightonP talk contribs uploaded File:Example word file.png (Screenshot of example Word file, before pandoc conversion)
- 13:17, 25 October 2019 User account CharlieBegley talk contribs was created by DaveMark talk contribs
- 13:16, 25 October 2019 User account MegJeppesen talk contribs was created by DaveMark talk contribs (New Member)
- 13:15, 25 October 2019 User account MollyKeithBaker talk contribs was created by DaveMark talk contribs (New Member)
- 13:14, 25 October 2019 User account ElmiraM talk contribs was created by DaveMark talk contribs
- 15:16, 17 October 2019 Alicia talk contribs created page Site-specific phage recombinase (Created page with "<strong>Integration sites</strong> You can check the sequence of your organism to see if it contains the appropriate integration site for your chosen plasmid: *ΦBT1, attB...")
- 15:44, 10 October 2019 J Newitt talk contribs created page Links master page (Created page with "=Useful Links= ==Bioinformatics== ===16S rRNA metaprofiling=== [https://greengenes.secondgenome.com Green Genes] 16S rRNA gene database [https://www.arb-silva.de/ SILVA]...")
- 16:25, 30 September 2019 DaveMark talk contribs created page Aminoglycosides (Created page with "=Intro= The aminoglycoside antibiotics are a class of amino containing oligosaccharides, and are notable for their broad-spectrum bacteriocidal activity - including their abil...")
- 14:35, 27 September 2019 LisAlgora talk contribs created page Southern Blotting (Created page with "There are many different protocols for Southern blotting. This approach uses vacuum transfer pf DNA to a nylon membrane, DIG-labelling of the probe and detection by chromogeni...")
- 13:53, 27 September 2019 EmilyAddington talk contribs created page Actinomycetes Morphology and Lifecycle (Created blank page)
- 08:57, 27 September 2019 User account JoshKlein talk contribs was created by MFeeney talk contribs and password was sent by email
- 08:56, 27 September 2019 User account LouCharkoudian talk contribs was created by MFeeney talk contribs and password was sent by email
- 16:01, 26 September 2019 J Newitt talk contribs created page General Growth and Maintenance of Streptomyces sp. (Created page with "=General growth and maintenance of ''Streptomyces'' species= ''Streptomyces'' are multi-nuclear filamentous organisms that undergo several different stages of growth. This se...")
- 15:25, 26 September 2019 Tommclean talk contribs created page Luria (Created page with "=Luria Broth= Known as Luria Broth (or sometimes, mistakenly, as LB or Lennox medium), this rich medium is used routinely for growth of <em>Escherichia coli</em>. There are se...")
- 15:25, 26 September 2019 Sam Prudence talk contribs created page File:SCOR2.jpg
- 15:25, 26 September 2019 Sam Prudence talk contribs uploaded File:SCOR2.jpg
- 15:23, 26 September 2019 Tommclean talk contribs created page Lennox (Created page with "=Lennox Broth= Known as Lennox Broth (or sometimes, mistakenly, as Luria-Bertani medium), this rich medium is used routinely for growth of <em>Escherichia coli</em>. There are...")
- 15:12, 26 September 2019 Tommclean talk contribs created page R5 (Created page with "=R5= ==Preparation== Make up the following solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *10.12 g MgCl<sub>2</sub>.6H<sub>2</sub>O *10 g Glucose *0.1 g Difco...")
- 15:57, 12 September 2019 Sam Prudence talk contribs created page Isolating extracellular protein from Streptomyces (Created page with " =Isolation of extracellular protein from ''Streptomyces''= This protocol can be used to isolate membrance associated and secreted extracellular proteins from ''Streptomyces''...")
- 09:15, 11 September 2019 Sam Prudence talk contribs created page Test (Created page with "| rowspan="8" valign="top" width="120" | texty wexty ! colspan="2" align="left" | <font face="Arial Black" size="5" style="text-transform: uppercase;"> Heading A </font> | row...")
- 13:20, 4 September 2019 LisAlgora talk contribs created page Trace element solution (Created page with "'''Trace element solution (per liter):''' *40 mg ZnCl<sub>2</sub> *200 mg FeCl<sub>3</sub> . 6H<sub>2</sub>O *10 mg CuCl<sub>2</sub> . 2H<sub>2</sub>O *10 mg MnCl<sub>2</sub>...")
- 13:17, 4 September 2019 LisAlgora talk contribs created page L buffer (Created page with "'''L (lysis) buffer''' (Thompson ''et al.'' 1982) Mix the following sterile solutions: *100 ml Sucrose (10.3%) *10 ml TES buffer (5.73%, adjusted to pH7.2) *1 ml K<sub>2</su...")
- 12:51, 4 September 2019 LisAlgora talk contribs created page P buffer (Created page with "'''P (protoplast) buffer''' (Okanishi ''et al.'', 1974; Hopwood and Wright, 1978) Make up the following basal solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *2....")
- 09:15, 4 September 2019 User account D Guillen talk contribs was created by Sam Prudence talk contribs
- 14:37, 2 September 2019 User account LeightonP talk contribs was created by MFeeney talk contribs and password was sent by email
- 04:37, 31 August 2019 H Otani talk contribs created page Rhodococcus jostii (Created page with "==Organism Page== === ''Rhodococcus jostii'' === ===Background=== ===Growth & Maintenance=== LB or M9 =====Sporulation===== Nonsporulating ===Strains=== <em>Rh...")
- 00:21, 31 August 2019 H Otani talk contribs created page Rhodococcus (Created page with "Rhodococci are nonsporulating actinomycetes in the family Nocardiaceae along with <em>Nocardia</em>. They are known for their ability to catabolise a wide range of organic...")
- 20:48, 30 August 2019 H Otani talk contribs created page Saccharopolyspora spinosa (Created page with "==Organism Page== === ''Saccharopolyspora spinosa'' === ===Background=== <em>Saccharopolyspora spinosa</em> is known for producing an insecticide, spinosad. Spinosad is a m...")
- 20:37, 30 August 2019 H Otani talk contribs created page YMPD (Created page with "=YMPD (Yeast Meat Peptone Dextrose)= For growth of ''Streptomyces griseus''. ==Preparation== Per litre: *2 g yeast extract *2.2 g meat extract *4 g Bacto peptone *5 g Na...")
- 15:40, 30 August 2019 EmilyAddington talk contribs uploaded a new version of File:Lifecycle.jpeg
- 15:36, 30 August 2019 EmilyAddington talk contribs created page File:Lifecycle.jpeg
- 15:36, 30 August 2019 EmilyAddington talk contribs uploaded File:Lifecycle.jpeg
- 15:28, 30 August 2019 LisAlgora talk contribs created page Protoplasts Formation, Regeneration and Transformation (Created page with "Protoplasts are used for fusion, transformation and transfection. =Protoplasts Preparation= Although this protocol was written for ''Streptomyces lividans'' and ''Str...")
- 15:03, 30 August 2019 ASBirke talk contribs created page Streptomyces clavuligerus (Created page with "=''Streptomyces clavuligerus''= ''Streptomyces clavuligerus'' was originally isolated from a South American soil sample for its ability to produce cephalosporins. Tod...")
- 15:01, 30 August 2019 DaveMark talk contribs created page File:Oligo 3.png
- 15:01, 30 August 2019 DaveMark talk contribs uploaded File:Oligo 3.png
- 14:40, 30 August 2019 MFeeney talk contribs created page Random mutagenesis using a mutator strain (Created page with "=<strong>Random mutagenesis using a mutator strain</strong>= This protocol has been confirmed to work for the following organisms. *<em>Streptomyces coelicolor</em> ==P...")
- 14:32, 30 August 2019 REMcHugh talk contribs created page Conjugation using ET12567/pUZ8002 (Created page with "#Transform ET12467/pUZ8002 with oriT-containing vector, and select for the incoming plasmid only #Inoculate a colony in to 10 ml LB containing Cm, Km and antibiotic used to se...")
- 13:57, 30 August 2019 EmilyAddington talk contribs created page Actinobacteria (Created page with "=Actinobacteria= ==General== ==Taxonomy== ==Ecology== ==Morphology and Lifecycle== ==Actinobacteria genetics== ==Antimicrobial production== ==References== ==Further Reading==")
- 13:53, 30 August 2019 LisAlgora talk contribs created page File:PIJ6902.gif
- 13:53, 30 August 2019 LisAlgora talk contribs uploaded File:PIJ6902.gif
- 13:48, 30 August 2019 LisAlgora talk contribs created page PIJ6902 (Created page with "{{DISPLAYTITLE: pIJ6092}} ==Use== ==Features== *Apramycin resistance cassette *φBT1 integration site & integrase ==History== ==Map== ==Sequence links== ==References=...")
- 13:47, 30 August 2019 User account EmilyAddington talk contribs was created by DaveMark talk contribs
- 13:20, 30 August 2019 User account Aros050 talk contribs was created by MFeeney talk contribs
- 10:52, 30 August 2019 User account D Udwary talk contribs was created by Sam Prudence talk contribs
- 10:48, 30 August 2019 User account H Otani talk contribs was created by Sam Prudence talk contribs
- 15:35, 29 August 2019 Tommclean talk contribs created page Exploration (Created page with "==General overview== <sup>[1]</sup> <strong>Useful host strains:</strong> *<em>Streptomyces venezuelae </em> <sup>[1]</sup> This species is currently the most well character...")
- 15:13, 29 August 2019 Tommclean talk contribs created page Characterisation of Actinomycetes (Created page with "Exploration Production of microbial volatile organic compounds")
- 14:50, 29 August 2019 User account Xtang talk contribs was created by Sam Prudence talk contribs
- 06:27, 29 August 2019 Yvonne Mast talk contribs created page Genome-based taxonomic analysis (Created page with "[https://tygs.dsmz.de/ TYGS] Type (Strain) Genome Server")
- 15:26, 27 August 2019 MFeeney talk contribs created page Teaching and Learning Resources: Antibiotics and AMR (Created page with "=Teaching and Learning Resources: Antibiotics and AMR= This section is our spotlight on teaching resources aimed for teaching about antibiotics and antimicrobial resistance at...")
- 11:14, 26 August 2019 Sam Prudence talk contribs protected Contribution Guidelines [Edit=Allow only administrators] (indefinite) [Move=Allow only administrators] (indefinite) (hist)
- 16:01, 20 August 2019 MFeeney talk contribs created page Mutagenesis (Created page with "A number of different methods have been developed for random and directed mutagenesis in actinobacteria (most notably for <em>Streptomyces coelicolor</em>). Many involve an in...")
- 16:25, 15 August 2019 S Worsley talk contribs created page RNA extraction from Actinobacteria (Created page with " =UNFINISHED PAGE: material is still being added= =Summary= This protocol for RNA extraction can be used to isolate large yields of high quality RNA from actinobacterial strai...")
- 15:12, 15 August 2019 User account S Worsley talk contribs was created by Sam Prudence talk contribs
- 10:34, 15 August 2019 Sam Prudence talk contribs created page File:Microsoc logo.png
- 10:34, 15 August 2019 Sam Prudence talk contribs uploaded File:Microsoc logo.png
- 14:44, 14 August 2019 J Newitt talk contribs created page Isolating Streptomyces from plant roots (Created page with "{{DISPLAYTITLE: Isolating ''Streptomyces'' from plant roots}}")
- 14:39, 14 August 2019 J Newitt talk contribs created page Isolating Actinomycetes from the environment (Created page with "Isolating ''Streptomyces'' from plant roots")
- 13:56, 13 August 2019 Alicia talk contribs uploaded a new version of File:PKC1132 Map.png
- 13:55, 13 August 2019 Alicia talk contribs created page File:PKC1132 Map.png
- 13:55, 13 August 2019 Alicia talk contribs uploaded File:PKC1132 Map.png
- 10:11, 13 August 2019 Andyxmas talk contribs created page Map (Created page with "<html> <iframe src="https://www.google.co.uk/maps/d/u/0/embed?mid=14bRNmsDo2vIXP_I8CVOgW4DJGB7ewkuf" width="640" height="480"></iframe> </html>")
- 09:50, 13 August 2019 Sam Prudence talk contribs moved page Salting Out DNA Extraction Method to Salting Out Genomic DNA Extraction Method
- 09:50, 13 August 2019 Sam Prudence talk contribs deleted page Salting Out Genomic DNA Extraction Method (Deleted to make way for move from "Salting Out DNA Extraction Method")
- 09:49, 13 August 2019 Sam Prudence talk contribs moved page Salting out method to Salting Out Genomic DNA Extraction Method
- 09:48, 13 August 2019 Sam Prudence talk contribs moved page Salting out method to Salting Out DNA Extraction Method
- 19:47, 12 August 2019 J Newitt talk contribs created page Salting out method (Created page with "=Isolation of high molecular weight (HMW) genomic DNA by salting out= The salting out method is used to isolate high yields of high molecular weight DNA, usually for applicat...")
- 08:22, 12 August 2019 Sam Prudence talk contribs moved page PCRISPomyces2 to PCRISPomyces-2
- 09:32, 9 August 2019 Alicia talk contribs created page File:PIJ10257 Map2.png
- 09:32, 9 August 2019 Alicia talk contribs uploaded File:PIJ10257 Map2.png
- 19:08, 8 August 2019 DaveMark talk contribs created page Contribution Guidelines (Created page with "==Intro==")
- 17:17, 8 August 2019 Alicia talk contribs created page File:PIJ10257 Map.png
- 17:17, 8 August 2019 Alicia talk contribs uploaded File:PIJ10257 Map.png
- 16:52, 8 August 2019 Sam Prudence talk contribs created page File:PCRISPomyces-2 map.png
- 16:52, 8 August 2019 Sam Prudence talk contribs uploaded File:PCRISPomyces-2 map.png
- 16:45, 8 August 2019 Sam Prudence talk contribs created page File:PCAP03 map.png
- 16:45, 8 August 2019 Sam Prudence talk contribs uploaded File:PCAP03 map.png
- 16:34, 8 August 2019 Sam Prudence talk contribs moved page PKT25 to PKT25/pKNT25
- 16:32, 8 August 2019 Sam Prudence talk contribs moved page PUT18 to PUT18/pUT18C
- 15:25, 8 August 2019 Sam Prudence talk contribs created page File:PRT802 Map.png
- 15:25, 8 August 2019 Sam Prudence talk contribs uploaded File:PRT802 Map.png
- 19:33, 3 August 2019 Sam Prudence talk contribs protected Test page [Create=Allow only administrators] (indefinite)
- 09:34, 3 August 2019 HeeeenaJ talk contribs created page ChIP Seq (Created page with "Buffers:- IP Buffer 50 mM HEPES pH to 7.5 with KOH 150 mM NaCl 1 mM EDTA 1 % Triton X-100 0.1 % Sodium deoxycholate 0.1 % SDS 5X Elution Buffer TE pH 7.6 5% SDS IP Buffer +...")
- 20:14, 2 August 2019 HeeeenaJ talk contribs created page PIJ12742 (Background)
- 12:45, 2 August 2019 Tommclean talk contribs created page Recruitment (Created page with "=Recruitment= Please feel free to post information and links to job opportunities etc below: *Please also remember to remove the links once filled.")
- 12:03, 2 August 2019 Matth talk contribs created page Groups (Created page with "Alphabetical list of research groups working on Streptomyces and closely related bacteria:")
- 10:35, 2 August 2019 User account R Devine talk contribs was created by Sam Prudence talk contribs
- 08:53, 2 August 2019 Sam Prudence talk contribs moved page Cloning and Expression of Gene Clusters to Cloning, Transformation and Expression of Gene Clusters
- 18:05, 1 August 2019 Sam Prudence talk contribs created page Tri-parental mating (Created page with "=<strong>Tri-parental Mating</strong>= ===Why Tri-Parental Mating?=== Tri-parental mating is useful for conjugating plasmids containing a kanamycin resistane gene as a selecta...")
- 15:57, 1 August 2019 Tommclean talk contribs created page SMMS (Created page with "=SMMS (Supplemented liquid minimal media, solid)= SMM is a solid supplemented minimal medium suitable for growth of many ''Streptomyces'' sp. ==Preparation== Per litre: *81.9...")
- 15:56, 1 August 2019 Jacob Hamilton talk contribs created page SPY (Created page with "=Salt Peptone Yeast Agar (SPY)= Salt Peptone Yeast agar is a metal-rich media used to stimulate the activation of cryptic biosynthetic gene clusters as part of antibiotic dis...")
- 15:55, 1 August 2019 Lucasbalis talk contribs created page Protein Methods (Created page with "Codon Optimising Sequences for Heterologous Expression in Streptomyces")
- 15:38, 1 August 2019 Jacob Hamilton talk contribs moved page Potato Dextrose Agar PDA to PDA (Rename)
- 15:36, 1 August 2019 Jacob Hamilton talk contribs moved page PGA to Potato Dextrose Agar PDA (Renaming from glucose to dextrose to maintain consistency)
- 15:33, 1 August 2019 Jacob Hamilton talk contribs created page PGA (Created page with "=Potato Dextrose Agar (PDA)= Potato Dextrose Agar is primarly used for the growth of the opportunistic fungal pathogen ''Scedosporium prolificans''. Thus, it is mostly used...")
- 15:30, 1 August 2019 Tommclean talk contribs created page SMM (Created page with "=SMM (Supplemented liquid minimal media)= Strauch et al., 1991<sup>1</sup> ==Preparation== Per litre: *81.9 mL PEG 6000 (BDH 6.1% w/v in distilled water) *2.5 mL Mg<sub>2</su...")
- 15:24, 1 August 2019 MFeeney talk contribs created page Overview of Streptomyces vectors (Created page with "150px|Plasmids|link=Plasmids =Commonly Used Streptomyces Vectors= This page describes the characteristics of some of the most co...")
- 15:21, 1 August 2019 Alicia talk contribs created page Streptomyces coelicolor A3(2) (Created page with "==Organism Page== === ''Streptomyces coelicolor A3(2)'' === ===Background=== ===Growth & Maintenance=== =====Sporulation===== *Sporulation can be achieved after 3-5 days gr...")
- 11:00, 1 August 2019 MFeeney talk contribs created page HT (Created page with "=Hickey-Tresner agar (HT)= This medium was developed by Hickey and Tresner<sup>[1]</sup> for the growth of some ''Streptomyces'' sp. that did not sporulate well on Bennett's...")
- 13:39, 31 July 2019 MFeeney talk contribs created page Antibiotic stocks and working concentrations (Created page with "{| class="wikitable" ! style="font-weight:bold; background-color:#c0c0c0;" | Antibiotic ! style="font-weight:bold; background-color:#c0c0c0;" | Stock [mg/ml] ! style="font-wei...")
- 13:29, 31 July 2019 User account Paulhoskisson talk contribs was created by MFeeney talk contribs and password was sent by email
- 11:06, 31 July 2019 MFeeney talk contribs created page CM (Created page with "=CM (Complete Medium)= ==Preparation== Per litre: *5g K<sub>2</sub>HPO<sub>4</sub> *0.5g NaCl *0.5g MgSO<sub>4</sub>*7H<sub>2</sub>O *2g Difco Bacto-peptone *1.5g Difco Casa...")
- 08:21, 30 July 2019 MFeeney talk contribs created page IMA (Created page with "=IMA(Instant Mash Agar)= IMA is sometimes also called [https://en.wikipedia.org/wiki/Smash_(instant_mashed_potato) Smash] (after the brand name of the instant potato commonly...")
- 20:47, 29 July 2019 User account HeeeenaJ talk contribs was created by MFeeney talk contribs and password was sent by email
- 10:59, 29 July 2019 CraigAllan talk contribs created page Streptomyces griseus (Created page with "==Organism Page== === ''Streptomyces griseus'' === ===Background=== ===Growth & Maintenance=== =====Sporulation===== ===Strains=== ====Superhosts==== ===Links=== =====Ge...")
- 10:47, 29 July 2019 ActinoCommittee talk contribs changed group membership for CraigAllan from administrator to administrator, interface administrator and bureaucrat
- 10:46, 29 July 2019 ActinoCommittee talk contribs changed group membership for CraigAllan from (none) to administrator
- 10:46, 29 July 2019 User account CraigAllan talk contribs was created by ActinoCommittee talk contribs (Actino Committee)
- 10:07, 29 July 2019 MFeeney talk contribs created page NMMP (Created page with "=Minimal liquid medium (NMMP)= NMMP medium is used to obtain dispersed growth of ''Streptomyces'' sp. with specific carbon sources. ==Preparation== Make up the following sol...")
- 15:12, 28 July 2019 DaveMark talk contribs created page ISP7 (Created page with "=ISP 7= ==Preparation== *15 g Glycerol *0.5 g L-tyrosine *1 g L-asparagine *0.5 g K<sub>2</sub>HPO<sub>4</sub> *0.5 g MgSO<sub>4</sub> x 7H<sub>2</sub>O *0.5 g NaCl *0.01 g...")
- 13:24, 28 July 2019 DaveMark talk contribs created page ISP6 (Created page with "=ISP6= ==Preparation== *36 g Bacto-Peptone Iron Agar (Difco) **15 g Bacto-Peptone **5 g Proteose-peptone ** 0.5 g Ferric ammonium citrate ** 1g K<sub>2</sub>2HPO<sub>4</sub...")
- 12:28, 28 July 2019 DaveMark talk contribs created page File:ISP 6 well.png (6-well plate with ISPs 2-7)
- 12:28, 28 July 2019 DaveMark talk contribs uploaded File:ISP 6 well.png (6-well plate with ISPs 2-7)
- 17:16, 26 July 2019 LisAlgora talk contribs created page R2YE (Created page with "=R2YE= R2YE medium is a variation of R2 containing yeast extract. ==Preparation== Make up the following solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *10.12 g...")
- 16:01, 26 July 2019 LisAlgora talk contribs created page R2 (Created page with "=R2= ==Preparation== #Make up the following solution: *103 g Sucrose *0.25 g K<sub>2</sub>SO<sub>4</sub> *10.12 g MgCl<sub>2</sub>.6H<sub>2</sub>O *10 g Glucose *0.1 g Difc...")
- 15:57, 26 July 2019 Laia talk contribs moved page Category:Actinobacteria isolation from marine environments to Actinobacteria isolation from marine environments over redirect
- 15:57, 26 July 2019 Laia talk contribs deleted redirect Actinobacteria isolation from marine environments by overwriting (Deleted to make way for move from "Category:Actinobacteria isolation from marine environments")
- 15:55, 26 July 2019 Laia talk contribs moved page Actinobacteria isolation from marine environments to Category:Actinobacteria isolation from marine environments
- 15:52, 26 July 2019 MFeeney talk contribs created page PUT18C (Created page with "{{DISPLAYTITLE: pUT18C}} =pUT18C= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:51, 26 July 2019 MFeeney talk contribs created page PUT18 (Created page with "{{DISPLAYTITLE: pUT18}} =pUT18= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:51, 26 July 2019 MFeeney talk contribs created page PKT25 (Created page with "{{DISPLAYTITLE: pKT25}} =pKT25= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:50, 26 July 2019 MFeeney talk contribs created page PKNT25 (Created page with "{{DISPLAYTITLE: pKNT25}} =pKNT25= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:50, 26 July 2019 Jordanmurray talk contribs created page Media (Redirected page to Media Recipes) Tag: New redirect
- 15:50, 26 July 2019 MFeeney talk contribs created page PET28a (Created page with "{{DISPLAYTITLE: pET28a}} =pET28a= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:49, 26 July 2019 MFeeney talk contribs created page PIJ773 (Created page with "{{DISPLAYTITLE: pIJ773}} =pIJ773= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:49, 26 July 2019 MFeeney talk contribs created page PSS170 (Created page with "{{DISPLAYTITLE: pSS170}} =pSS170= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:48, 26 July 2019 MFeeney talk contribs created page PRT802 (Created page with "{{DISPLAYTITLE: pRT802}} =pRT802= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:47, 26 July 2019 MFeeney talk contribs created page PMF96 (Created page with "{{DISPLAYTITLE: pMF96}} =pMF96= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:47, 26 July 2019 MFeeney talk contribs created page PKC1132 (Created page with "{{DISPLAYTITLE: pKC1132}} =pKC1132= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:46, 26 July 2019 MFeeney talk contribs created page PIJ12738 (Created page with "{{DISPLAYTITLE: pIJ12738}} =pIJ12738= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:45, 26 July 2019 MFeeney talk contribs created page PIJ8660 (Created page with "{{DISPLAYTITLE: {{DISPLAYTITLE: pIJ10257}} =pIJ8660= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:43, 26 July 2019 ActinoCommittee talk contribs created page Metabolite extractions (Protocols to extract metabolites from Actinobacteria)
- 15:38, 26 July 2019 MFeeney talk contribs created page PIJ733 (Created page with "{{DISPLAYTITLE: pIJ773}} =pIJ773= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:37, 26 July 2019 DaveMark talk contribs created page ISP5 (Created page with "=ISP 5= ==Preparation== *1 g L-asparagine (anhydrous) *10 g Glycerol *1 g K<sub>2</sub>HPO<sub>4</sub> *1 L dH<sub>2</sub>O *1 mL Trace salts solution **0.1 g FeSO<sub>4</s...")
- 15:27, 26 July 2019 REMcHugh talk contribs created page File:Sgoldwiki.png
- 15:27, 26 July 2019 REMcHugh talk contribs uploaded File:Sgoldwiki.png
- 15:22, 26 July 2019 DaveMark talk contribs created page ISP4 (Created page with "=ISP 4= International <i>Streptomyces</i> Project medium 4, also named inorganic salts-starch agar ==Preparation== *10 g Soluble Starch *1 g MgSO<sub>4</sub> x 7H<sub>2</sub>...")
- 15:19, 26 July 2019 REMcHugh talk contribs created page File:Sgoldiniensis.png
- 15:19, 26 July 2019 REMcHugh talk contribs uploaded File:Sgoldiniensis.png
- 15:15, 26 July 2019 MFeeney talk contribs created page PIJ10257 (Created page with "{{DISPLAYTITLE: pIJ10257}} =pIJ10257= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 15:11, 26 July 2019 REMcHugh talk contribs created page File:Streptomyces goldiniensis.png
- 15:11, 26 July 2019 REMcHugh talk contribs uploaded File:Streptomyces goldiniensis.png
- 15:09, 26 July 2019 ASBirke talk contribs created page MC agar (Created page with "=MC agar= This media is used to screen Streptomyces strains for extracellular protease production and the resulting degradation of the milk powder components on the plate, wh...")
- 15:09, 26 July 2019 Jordanmurray talk contribs created page Staining of ''Streptomyces'' spp. Cell Wall and Nucleic Acids (Redirected page to Staining of Streptomyces spp. Cell Wall and Nucleic Acids) Tag: New redirect
- 15:09, 26 July 2019 User account LRooney talk contribs was created by MFeeney talk contribs (Additional user)
- 15:04, 26 July 2019 MFeeney talk contribs created page Supercos1 (Created page with " =SuperCos1= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 14:59, 26 July 2019 MFeeney talk contribs created page PESAC13 (Created page with "=pESAC13= ==Background== ==Construction== ==Uses== ==Characteristics== ==Map and Sequence== ==References==")
- 14:52, 26 July 2019 MFeeney talk contribs created page SC (Created page with "=Starch Casein Agar= ==Preparation== Per Litre: *10 g soluble starch *2g K<sub>2</sub>HPO<sub>4</sub> *2g KNO<sub>3</sub> *0.3g casein *0.05g MgSO<sub>4</sub>*7H<sub>2</sub>O...")
- 14:46, 26 July 2019 LisAlgora talk contribs created page SNA (Created page with "=SNA= Soft Nutrient Agar (SNA) ==Preparation== Per Litre: *4g Difco Nutrient Broth Powder *5g Agar *1L dH<sub>2</sub>O '''Instructions''' #Add 0.8g of DNB powder into eac...")
- 14:41, 26 July 2019 MFeeney talk contribs created page OT (Created page with "=OT(oligotrophic agar)= Oligotrophic agar is an extremely nutritionally-poor medium useful for isolating slow-growing actinomyces e.g. from soil. ==Preparation== Per litre:...")
- 14:36, 26 July 2019 REMcHugh talk contribs created page Streptomyces goldiniensis (Created page with "=<em>Streptomyces goldiniensis </em>= <em>Streptomyces goldiniensis </em>is a Gram-positive soil dwelling bacterium that was first isolated from the island of Bermuda in 1969...")
- 14:27, 26 July 2019 MFeeney talk contribs created page HV (Created page with "=HV= ==Preparation== Per Litre: *1g humic acid *1.7g KCl *0.5g Na<sub>2</sub>HPO<sub>4</sub> *0.5g MgSO<sub>4</sub> *0.02g CaCO<sub>3</sub> *0.01g FeSO<sub>4</sub> *1mL VB...")
- 14:24, 26 July 2019 LisAlgora talk contribs created page 2X YT (Created page with "=2x YT= ==Preparation== Per litre: *16 g Tryptone *10 g Yeast Extract *5 g NaCl *1 L dH<sub>2</sub>O '''Instructions''' #Dissolve constituents in 1L dH<sub>2</sub>O #Ste...")
- 14:18, 26 July 2019 DaveMark talk contribs created page ISP3 (Created page with "=ISP 3= International Streptomyces Project media 3 (ISP 3) is a rich general use media. Alternatively called oatmeal agar. ==Preparation== *20 g White Oats *18 g Agar *1 mL...")
- 14:18, 26 July 2019 Jordanmurray talk contribs created page Staining of Streptomyces spp. Cell Wall and Nucleic Acids (Created page with "The following staining technique is a useful method for visualising the actively growing regions of the cell wall, along with the nucleic acids within the cell. The method was...")
- 14:08, 26 July 2019 ActinoCommittee talk contribs created page Emerson medium (Created page with "=Emerson medium= Emerson medium is a rich medium formulated by Emerson et al (1) to isolate mould and Actinobacteria. It is composed of beef extract, yeast extract, peptic di...")
- 13:54, 26 July 2019 User account Jacob Hamilton talk contribs was created by Sam Prudence talk contribs
- 13:51, 26 July 2019 User account ASBirke talk contribs was created by MFeeney talk contribs
- 13:48, 26 July 2019 DaveMark talk contribs created page GYM (Created page with "=Glucose Yeast-Extract Malt-Extract Media= ==Preparation== Per litre: *4 g Glucose *4 g Yeast Extract *10 g Malt Extract *CaCo<sub>3</sub> *12 g Agar *1 L dH<sub>2</sub>O...")
- 13:47, 26 July 2019 User account REMcHugh talk contribs was created by MFeeney talk contribs
- 13:36, 26 July 2019 ActinoCommittee talk contribs moved page Environmental isolation to Actinobacteria isolation from marine environments
- 13:31, 26 July 2019 Jordanmurray talk contribs created page Microscopy (Created page with "=Microscopy of Actinobacteria= ==<em>Streptomyces</em>==")
- 13:25, 26 July 2019 User account RMcHugh talk contribs was created by MFeeney talk contribs
- 13:21, 26 July 2019 ActinoCommittee talk contribs created page Environmental isolation (Protocols to isolate Actinobacteria from different environments)
- 13:18, 26 July 2019 User account LisAlgora talk contribs was created by MFeeney talk contribs
- 13:17, 26 July 2019 User account Jordanmurray talk contribs was created by MFeeney talk contribs
- 13:00, 26 July 2019 User account MunnJ90 talk contribs was created by MFeeney talk contribs